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- Litvinenko, Yu.A., et al.
(författare)
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Nonlinear sinusoidal and varicose instability in the boundary layer (review)
- 2004
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Ingår i: Thermophysics and Aeromechanics. ; 11:3, s. 329-354
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Tidskriftsartikel (refereegranskat)abstract
- Results of studying sinusoidal and varicose instabilities of streaky structures at the nonlinear stage of the laminar-turbulent process in shear flows are presented. The flow behavior in the course of spatial evolution of streaky structures with a secondary high-frequency disturbance generated on them is discussed. Various scenarios of origination and development of coherent vortex structures examined in physical and numerical experiments are considered. Specific features of the development of sinusoidal and varicose cases of destruction of the steady streamwise streaky structure are demonstrated, such as transverse and streamwise modulation of the structure by the secondary-disturbance frequency, appearance of new streaky structures in the downstream direction, and emergence and evolution of unsteady Λ-type structures localized in space in both cases.
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| 3. |
- McCracken, Vance J, et al.
(författare)
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TNF-alpha Sensitizes HT-29 Colonic Epithelial Cells to Intestinal Lactobacilli.
- 2002
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Ingår i: Experimental Biology and Medicine. - 1535-3702. ; 227:8, s. 665-670
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Tidskriftsartikel (refereegranskat)abstract
- The ability of the proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) to influence epithelial interleukin (IL)-8 responses to the intestinal bacterium Lactobacillus plantarum 299v was analyzed in the human HT-29 colonic epithelial cell line. In the absence of TNF-alpha, IL-8 mRNA expression was not detectable by Northern blot analysis in HT-29 cells alone or in HT-29 cells co-cultured with L. plantarum 299v. However, TNF-alpha induced IL-8 mRNA expression, and co-culture of TNF-alpha-treated HT-29 cells with L. plantarum 299v significantly increased IL-8 mRNA expression above levels induced by TNF-alpha alone in an adhesion-dependent manner. The increase in IL-8 mRNA expression was not observed in TNF-alpha-treated HT-29/L. plantarum 299v co-cultures using heat-killed lactobacilli or when L. plantarum adhesion was prevented using mannoside or a trans-well membrane. Paradoxically, IL-8 secretion was decreased in TNF-alpha-treated HT-29 cells with L. plantarum 299v relative to cells treated with TNF-alpha alone. TNF-alpha-mediated responsiveness to L. plantarum 299v was further investigated by analyzing expression of a coreceptor for bacterial cell wall products CD14. HT-29 cells expressed CD14 mRNA and cell-surface CD14; however, TNF-alpha did not alter CD14 mRNA or cell-surface expression, and blockade of CD14 with monoclonal antibody MY4 did not alter the IL-8 response to L. plantarum 299v in TNF-alpha-treated HT-29 cells. These results indicate that although TNF-alpha sensitizes HT-29 epithelial cells to intestinal lactobacilli, the bacteria exert a protective effect by downregulating IL-8 secretion.
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