| 1. |
- Orsato, Renato, et al.
(författare)
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The political ecology of automobile recycling in Europe
- 2002
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Ingår i: Organization Studies. - : SAGE Publications. - 1741-3044 .- 0170-8406. ; 23:4, s. 639-665
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Tidskriftsartikel (refereegranskat)abstract
- This paper addresses the relationship between organizations and the natural environment from both theoretical and empirical perspectives. In doing so, it contributes in three ways. First, it satisfies the need for more political perspectives in enviromnent-related research. Second, by analyzing the end-of-life vehicle issue that the European automobile industry addressed in the 1990s, the paper satisfies the need of developing research that integrates organizational and field-level analysis. Finally, the use of the political ecology framework for the analysis of the end-of-life vehicle issue contributes to the development of a more politically charged institutional theory in which, as the study shows, both inertia and change in organizational fields depend on circuits of political ecology.
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| 2. |
- Webster, Matthew, et al.
(författare)
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Common 5' beta-globin RFLP haplotype harbour a surprising level of ancestral sequence mosaicism
- 2003
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Ingår i: Human Genetics. - : Springer Science and Business Media LLC. - 0340-6717 .- 1432-1203. ; 113:2, s. 123-139
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Blocks of linkage disequilibrium (LD) in the human genome represent segments of ancestral chromosomes. To investigate the relationship between LD and genealogy, we analysed diversity associated with restriction fragment length polymorphism (RFLP) haplotypes of the 5' beta-globin gene complex. Genealogical analyses were based on sequence alleles that spanned a 12.2-kb interval, covering 3.1 kb around the psibeta gene and 6.2 kb of the delta-globin gene and its 5' flanking sequence known as the R/T region. Diversity was sampled from a Kenyan Luo population where recent malarial selection has contributed to substantial LD. A single common sequence allele spanning the 12.2-kb interval exclusively identified the ancestral chromosome bearing the "Bantu" beta(s) (sickle-cell) RFLP haplotype. Other common 5' RFLP haplotypes comprised interspersed segments from multiple ancestral chromosomes. Nucleotide diversity was similar between psibeta and R/T-delta-globin but was non-uniformly distributed within the R/T-delta-globin region. High diversity associated with the 5' R/T identified two ancestral lineages that probably date back more than 2 million years. Within this genealogy, variation has been introduced into the 3' R/T by gene conversion from other ancestral chromosomes. Diversity in delta-globin was found to lead through parts of the main genealogy but to coalesce in a more recent ancestor. The well-known recombination hotspot is clearly restricted to the region 3' of delta-globin. Our analyses show that, whereas one common haplotype in a block of high LD represents a long segment from a single ancestral chromosome, others are mosaics of short segments from multiple ancestors related in genealogies of unsuspected complexity.
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| 3. |
- Webster, Matthew T, et al.
(författare)
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Analysis of variation in the human beta-globin gene cluster using a novel DHPLC technique.
- 2002
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Ingår i: Mutation research. - 0027-5107 .- 1873-135X. ; 501:1-2, s. 99-103
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Tidskriftsartikel (refereegranskat)abstract
- We have implemented a technique combining allele-specific PCR (AS-PCR) and denaturing high-performance liquid chromatography (DHPLC) to identify new polymorphic variants within an intergenic region in the beta-globin cluster. This technique is applicable to the detection of new variants in genomic regions where variation is apportioned into distinct classes of haplotype. Duplexes for DHPLC analysis were created by denaturation and re-annealing of a mixture of two AS-PCR products of known and unknown sequence from the same haplotypic class, permitting detection of new haplotypes in each class. A 454bp fragment 3.5kb 5' to the human delta-globin gene, which may have a gene regulatory function, was analysed in 840 chromosomes from a global sampling of human populations using this method. Two divergent haplotypes were found to predominate in all populations studied, possibly as a result of balancing selection.
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