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Träfflista för sökning "WFRF:(Csöregi Elisabeth) srt2:(2006)"

Search: WFRF:(Csöregi Elisabeth) > (2006)

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1.
  • Mieliauskiene, R, et al. (author)
  • Amperometric determination of acetate with a tri-enzyme based sensor
  • 2006
  • In: Sensors and Actuators B: Chemical. - : Elsevier BV. - 0925-4005. ; 113:2, s. 671-676
  • Journal article (peer-reviewed)abstract
    • The present work describes a flow injection analysis system incorporating amperometric biosensors for acetate monitoring. Tri-enzyme-modified electrodes were fabricated by immobilizing acetate kinase (AK), pyruvate kinase (PK) and lactate dehydrogenase (LDH) into a poly(ethyleneglycole) diglycidyl ether (PEGDGE) film also containing Brilliant Cresyl Blue (BCB) as an electrochemical mediator. The concentration of acetate could be determined from the decrease in the amperometric current at +50 mV versus Ag/AgCl. Parameters, such as composition of the biosensing film, operational conditions (e.g., applied potential, flow rate) were optimized. The optimized biosensor showed a linear response to acetate in the range of 0.2-8 mM with a detection limit of 0.13 mM (calculated as 3 SIN). The acetate content of different samples (wine and vinegar) could be reliably quantified with the described biosensor.
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2.
  • Mortari, Alessia, et al. (author)
  • Applications of protein-based capacitive biosensors for the detection of heavy-metal ions
  • 2006
  • In: 2006 International Conference on Nanoscience and Nanotechnology (IEEE Cat. No.06EX1411). - 1424404525 ; , s. 304-307
  • Conference paper (peer-reviewed)abstract
    • Three different methods of using protein-based capacitive biosensors for the detection of heavy-metal ions are presented. The metal-binding proteins SmtA, S100A12, MerP and four modified MerPs were immobilised as the bio-recognition element on self-assembled monolayer-modified gold electrodes. Capacitance was measured using potential square step or electrical impedance spectroscopy. The protein-metal interaction generated changes in capacitance mainly due to a protein conformational change.
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  • Result 1-2 of 2
Type of publication
conference paper (1)
journal article (1)
Type of content
peer-reviewed (2)
Author/Editor
Csöregi, Elisabeth (2)
Martin, D (1)
Nistor, Mihaela (1)
Mortari, Alessia (1)
Brown, N L (1)
Mieliauskiene, R (1)
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Laurinavicius, V (1)
Geczy, C. (1)
Coster, H.G.L. (1)
Valenzuela, S.M. (1)
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University
Lund University (2)
Language
English (2)
Research subject (UKÄ/SCB)
Natural sciences (2)
Year

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