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- Czolkos, Ilja, 1980, et al.
(författare)
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Flow control of thermotropic lipid monolayers
- 2011
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Ingår i: Soft Matter. - : Royal Society of Chemistry (RSC). - 1744-6848 .- 1744-683X. ; 7:15, s. 6926-6933
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Tidskriftsartikel (refereegranskat)abstract
- There is an increasing interest in using liquid crystalline media as mobile phases in two-dimensional nanofluidic systems. Their small-scale, reduced dimensionality, and plentiful opportunities for functionalisation render such phases advantageous. However, flow control has been difficult to achieve, as the wetting processes which drive area expansion are not dynamically controllable. Here, we report on temperature-controlled monolayer spreading of 1,2-dielaidoyl-sn-glycero-3-phosphoethanolamine (DEPE) on the hydrophobic substrates SU-8, and Teflon AF (amorphous fluoropolymer). The gel/liquid phase transition of DEPE at T(c) similar to 38 degrees C is exploited to toggle spreading of a molecular lipid film on SU-8. We observed that on Teflon AF, DEPE monolayer spreading occurs even below T(c), and exhibits strongly accelerated spreading above the phase transition temperature. Our results demonstrate that switching DEPE monolayer spreading on and off, or, alternatively, switching between fast and slow area expansion, is a feasible approach towards establishing control over lipid film flow in two-dimensional fluidic systems. We also present a chip-based device integrating a patterned surface for 2D-microfluidics and on-chip heating.
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| 2. |
- Czolkos, Ilja, 1980, et al.
(författare)
-
Molecular phospholipid films on solid supports
- 2011
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Ingår i: Soft Matter. - : Royal Society of Chemistry (RSC). - 1744-6848 .- 1744-683X. ; 7:10, s. 4562-4576
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Tidskriftsartikel (refereegranskat)abstract
- Phospholipid membranes are versatile structures for mimicking biological surfaces. Bilayer and monolayer membranes can be formed on solid supports, leading to enhanced stability and accessibility of the biomimetic molecular film. This has facilitated functional studies of membrane proteins and aided the development of membrane-based applications in, for example, biosensing, self-assembled reaction kinetics and catalysis. Assembly and preparation of lipid films on supporting surfaces is a challenging engineering task with the goal of fabricating mechanically, chemically and thermodynamically stable lipid membranes. In this review, the current state of the art of molecularly thin lipid layer fabrication is presented with an emphasis on support materials, film formation mechanisms, characterisation methods, and applications.
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