Sökning: WFRF:(Daniels A)
> (1990-1994) >
A novel radioligand...
A novel radioligand binding assay to determine diagnostic accuracy of isoform-specific glutamic acid decarboxylase antibodies in childhood IDDM
-
- Grubin, C. E. (författare)
- University of Washington
-
- Daniels, T. (författare)
- University of Washington
-
- Toivola, B. (författare)
- University of Washington
-
visa fler...
-
- Landin-Olsson, M. (författare)
- Lund University,Lunds universitet,Medicin/akutsjukvård, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Diabetes lab,Forskargrupper vid Lunds universitet,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University Research Groups
-
- Hagopian, W. A. (författare)
- University of Washington
-
- Li, L. (författare)
- University of Washington
-
- Karlsen, A. E. (författare)
- Hagedorn Research Institute
-
- Boel, E. (författare)
- Novo Nordisk A/S
-
- Michelsen, B. (författare)
- Hagedorn Research Institute
-
- Lernmark, Å (författare)
- Karolinska Institute,Karolinska University Hospital,University of Washington
-
visa färre...
-
(creator_code:org_t)
- 1994
- 1994
- Engelska.
-
Ingår i: Diabetologia. - 0012-186X. ; 37:4, s. 344-350
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Insulin-dependent diabetes mellitus (IDDM) is associated with autoreactivity against GAD but the diagnostic sensitivity (positivity in disease) and specificity (negativity in health) of isoform-specific GAD antibodies have yet to be defined in assay systems suitable for screening large number of samples. One set of IDDM patient (n=10) and control (n=50) standard sera were used to develop quantitative antibody assays with in vitro synthesized recombinant 35S-methionine-labelled GAD65 and GAD67, respectively, and protein A-Sepharose to separate free from antibody-bound ligand. Binding levels were not normally distributed (p<0.0001) and therefore, the diagnostic accuracy of GAD antibodies was analysed by the ROC plots in population-based, consecutively-diagnosed, recent onset, 0-14 year-old patients (n=105), and matched, healthy control subjects (n=157). The ROC plots showed that the diagnostic sensitivity of GAD65 antibodies was 77% and the specificity 92% compared with 8% and 98%, respectively for GAD67 antibodies. In the IDDM sera, GAD65 and GAD67 antibodies were concordant in 7% (6 of 81) and GAD65 antibodies and ICA in 89% (72 of 81) without a correlation between the autoantibody levels. Autoantibodies to recombinant human islet GAD65 are specific and sensitive markers for childhood IDDM in this immunoassay with in vitro synthesized 35S-methioninelabelled recombinant GAD.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Nyckelord
- autoimmunity
- diabetes mellitus
- diagnostic accuracy
- Glutamic acid decarboxylase
- islet cell antibodies
- receiver-operating characteristic plot
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Grubin, C. E.
-
Daniels, T.
-
Toivola, B.
-
Landin-Olsson, M ...
-
Hagopian, W. A.
-
Li, L.
-
visa fler...
-
Karlsen, A. E.
-
Boel, E.
-
Michelsen, B.
-
Lernmark, Å
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Endokrinologi oc ...
- Artiklar i publikationen
-
Diabetologia
- Av lärosätet
-
Lunds universitet