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| 2. |
- Söderlind, Eskil, et al.
(författare)
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Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries
- 2000
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Ingår i: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1087-0156 .- 1546-1696. ; 18:8, s. 852-856
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Tidskriftsartikel (refereegranskat)abstract
- We constructed a single-chain Fv antibody library that permits human complementarity-determining region (CDR) gene fragments of any germline to be incorporated combinatorially into the appropriate positions of the variable-region frameworks VH-DP47 and VL-DPL3. A library of 2 x 109 independent transformants was screened against haptens, peptides, carbohydrates, and proteins, and the selected antibody fragments exhibited dissociation constants in the subnanomolar range. The antibody genes in this library were built on a single master framework into which diverse CDRs were allowed to recombine. These CDRs were sampled from in vivo-processed gene sequences, thus potentially optimizing the levels of correctly folded and functional molecules, and resulting in a molecule exhibiting a lower computed immunogenicity compared to naive immunoglobulins. Using the modularized assembly process to incorporate foreign sequences into an immunoglobulin scaffold, it is possible to vary as many as six CDRs at the same time, creating genetic and funcfional variation in antibody molecules.
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| 3. |
- Ahnquist, Johanna, et al.
(författare)
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Utvärdering pågår!
- 2003
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Ingår i: Alkohol & Narkotika. - 0345-0732. ; 97:4-5, s. 47-48
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 4. |
- Andersson, Gabriella, et al.
(författare)
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Kvinnor i fysik
- 2004
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Ingår i: KOSMOS.
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 6. |
- Danielsson, Anna, 1957, et al.
(författare)
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Energy expenditure in stroke subjects walking with a carbon composite ankle foot orthosis.
- 2004
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Ingår i: Journal of rehabilitation medicine : official journal of the UEMS European Board of Physical and Rehabilitation Medicine. - : Medical Journals Sweden AB. - 1650-1977. ; 36:4, s. 165-8
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To measure walking speed and energy cost in patients with prior stroke with and without a carbon composite ankle foot orthosis. DESIGN: Within-group comparisons of 2 walking conditions. PARTICIPANTS: Convenience sample of 10 hemiparetic patients with a stroke at least 6 months earlier (average age 52 years) habituated to a carbon composite ankle foot orthosis. METHODS: Subjects walked on a treadmill at self-selected speed both with and without ankle foot orthosis for 5 minutes on each occasion. Energy expenditure was measured by breath-by-breath analysis and electrocardiography. Main outcome measures were walking speed, oxygen consumption, heart rate and energy cost per metre. RESULTS: Walking speed: without ankle foot orthosis 0.27 (SEM +/- 0.03) m/s, with 0.34 (+/- 0.06) m/s, difference 20%. Oxygen consumption: without ankle foot orthosis 8.6 (+/- 0.4) ml/kg/min, with 8.8 (+/- 0.5) ml/kg/min. Energy cost: without ankle foot orthosis 0.58 (+/- 0.07) ml/kg/m, with 0.51 (+/- 0.06) ml/kg/m, difference 12%. CONCLUSION: Use of a carbon composite ankle foot orthosis in patients with stroke may increase speed and decrease energy cost during walking.
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| 7. |
- Danielsson, Anna, 1957, et al.
(författare)
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Oxygen consumption during treadmill walking with and without body weight support in patients with hemiparesis after stroke and in healthy subjects
- 2000
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Ingår i: Archives of Physical Medicine and Rehabilitation. - 0003-9993 .- 1532-821X. ; 81, s. 953-7
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Tidskriftsartikel (refereegranskat)abstract
- Objective: To compare oxygen consumption during walking with body weight support (BWS) with oxygen consumption during unsupported treadmill walking. Design: Patient and reference group. Comparisons between two walking conditions within each group. Setting: Research laboratory of a university hospital. Participants: Nonrandom convenience sample of 9 hemiparetic and 9 healthy subjects, mean age of 56 and 57 years, respectively. Interventions: The subjects walked on a treadmill with 0% and 30% BWS at their self-selected and maximum walking speeds. The trials were performed twice. Main Outcome Measures: Ventilatory oxygen uptake (VO2) and heart rate were measured by computerized breath-by-breath analysis and electrocardiography. Results: VO2 was lower during walking with 30% BWS than during unsupported walking. At self-selected speed the Wilcoxon's signed rank p values were <.01 for both patients and reference group; at maximum velocity, p values were p <.02 for the patients and p <.05 for the reference group. Patients' heart rates were lower when they walked with 30% BWS than at 0% BWS, at both self-selected and maximum walking speeds (p <.05 and p <.02, respectively). Conclusions: The 30% body weight supported condition requires less oxygen consumption than full weight bearing. Treadmill training with BWS can be tolerated by patients with cardiovascular problems.
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