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- Jullesson, David, 1987, et al.
(författare)
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Impact of synthetic biology and metabolic engineering on industrial production of fine chemicals
- 2015
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Ingår i: Biotechnology Advances. - : Elsevier BV. - 0734-9750. ; 33:7, s. 1395-1402
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Forskningsöversikt (refereegranskat)abstract
- Industrial bio-processes for fine chemical production are increasingly relying on cell factories developed through metabolic engineering and synthetic biology. The use of high throughput techniques and automation for the design of cell factories, and especially platform strains, has played an important role in the transition from laboratory research to industrial production. Model organisms such as Saccharomyces cerevisiae and Escherichia coli remain widely used host strains for industrial production due to their robust and desirable traits. This review describes some of the bio-based fine chemicals that have reached the market, key metabolic engineering tools that have allowed this to happen and some of the companies that are currently utilizing these technologies for developing industrial production processes.
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| 2. |
- David, Florian, 1981, et al.
(författare)
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Advances in yeast genome engineering
- 2015
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Ingår i: FEMS Yeast Research. - : Oxford University Press (OUP). - 1567-1356 .- 1567-1364. ; 15:1
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Tidskriftsartikel (refereegranskat)abstract
- Genome engineering based on homologous recombination has been applied to yeast for many years. However, the growing importance of yeast as a cell factory in metabolic engineering and chassis in synthetic biology demands methods for fast and efficient introduction of multiple targeted changes such as gene knockouts and introduction of multistep metabolic pathways. In this review, we summarize recent improvements of existing genome engineering methods, the development of novel techniques, for example for advanced genome redesign and evolution, and the importance of endonucleases as genome engineering tools.
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