| 1. |
- Buttle, David J, et al.
(författare)
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Human sputum cathepsin B degrades proteoglycan, is inhibited by a2-macroglobulin and modulated by neutrophil elastase cleavage of cathepsin B precursor and cystatin C
- 1991
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Ingår i: Biochemical Journal. - 0264-6021. ; 276, s. 325-331
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Tidskriftsartikel (refereegranskat)abstract
- The high-Mr alkali-stable form of cathepsin B was purified from purulent human sputum. It was shown to solubilize proteoglycan monomer entrapped in polyacrylamide at a rate comparable with that of human lysosomal cathepsin B. Like the enzyme from lysosomes, sputum cathepsin B was bound by human alpha 2-macroglobulin, which inhibited its action on proteoglycan. Cystatin C in purulent sputum was shown to be the N-terminally truncated form generated by neutrophil elastase cleavage, and sputum cathepsin B was only weakly inhibited by recombinant cystatin C that had been cleaved by neutrophil elastase in vitro. Addition of neutrophil elastase to mucoid sputum led to a 5-fold increase in cathepsin B activity concomitant with a lowering in Mr of the cysteine proteinase from 40,000 to 37,000, i.e. the size of the active enzyme purified from purulent sputum. It is concluded that the high-Mr form of cathepsin B present in purulent sputum is a functional proteinase, unlike similar forms of the enzyme secreted by mammary gland in organ culture. The activity of cathepsin B in sputum is modulated by neutrophil elastase, by a combination of inhibitor inactivation and zymogen activation.
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| 2. |
- Agardh, Elisabet, et al.
(författare)
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A four-year follow-up study on the incidence of diabetic retinopathy in older onset diabetes mellitus
- 1994
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Ingår i: Diabetic Medicine. - 1464-5491. ; 11:3, s. 273-278
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Tidskriftsartikel (refereegranskat)abstract
- Out of 369 diabetic patients with an age at onset of diabetes > or = 30 years previously studied, 325 (88%) were included in an ophthalmological follow-up examination 4 years later. In patients treated with oral drugs at baseline, the incidence of any type of retinopathy was 30.8% and of severe retinopathy 5.7%. All patients who developed severe retinopathy received insulin during the follow-up period. At baseline, duration of diabetes, diastolic blood pressure, and signs of nephropathy (p < 0.05 in all cases) as well as degree of metabolic control (p < 0.01) differed between patients who developed retinopathy and those who did not. At follow-up, there were no longer any differences regarding degree of metabolic control and diastolic blood pressure. In patients treated with insulin at baseline, the incidence of any type of retinopathy was 41.0% and of severe retinopathy 16.1%. At baseline, duration of diabetes (p < 0.01), degree of metabolic control, and insulin dosage (p < 0.05 in both cases) differed between patients who developed retinopathy and those who did not. At follow-up, there was no longer any difference in insulin dosage.
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| 3. |
- Hocherman, Irit, et al.
(författare)
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Monitoring traffic enforcement effectiveness on a national scale
- 1994
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Ingår i: Proceedings of Strategic Highway Research Program (SHRP) and traffic safety on two continents. Conference in Hague, Netherlands, September 22-24, 1993. - Linköping : Statens väg- och transportforskningsinstitut. ; , s. 247-268
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 4. |
- Magnusson, Peter S., et al.
(författare)
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A Compact Intermediate Format for SimICS
- 1994. - 1
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Instruction set architecture (ISA) simulators are an increasingly popular class of tools for both research and commercial purposes. Common applications include trace generation, program development, and compatibility support. A major concern with ISA simulators is performance and memory overhead. A common technique for achieving good performance is to use threaded code, which involves translating the target object code to an intermediate format which is subsequently interpreted. We describe such an internal format, which we call the 64-bit format, that is compact and meets a range of requirements in terms of flexibility and simplicity. We show how a simulator using this format can be implemented efficiently by taking advantage of extensions to the C language supported by the GNU C compilers. We have used the format to write the core interpreter in SimICS, a system level multiprocessor simulator that supports the Motorola 88110 and the SPARC V8 instruction sets.
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| 5. |
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