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PARP-1 gene disrupt...
PARP-1 gene disruption in mice preferentially protects males from perinatal brain injury
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- Hagberg, Henrik, 1955 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kvinnors och barns hälsa, Avdelningen för obstetrik och gynekologi,Institute for the Health of Women and Children, Dept of Obstetrics and Gynaecology
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Wilson, Mary Ann (författare)
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Matsushita, Hiroko (författare)
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- Zhu, Changlian, 1964 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi,Institute of Physiology and Pharmacology, Dept of Physiology
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Lange, Mary (författare)
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- Gustavsson, Malin, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi,Institute of Physiology and Pharmacology, Dept of Physiology
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Poitras, Marc F. (författare)
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Dawson, Ted. M. (författare)
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Dawson, Valina. L. (författare)
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Northington, Frances. (författare)
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Johnston, Michael. V. (författare)
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(creator_code:org_t)
- 2004
- 2004
- Engelska.
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Ingår i: J Neurochem. ; 90:5, s. 1068-75
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Poly(ADP-ribose) polymerase-1 is over-activated in the adult brain in response to ischemia and contributes to neuronal death, but its role in perinatal brain injury remains uncertain. To address this issue, 7-day-old wild-type (wt) and PARP-1 gene deficient (parp+/- and parp-/-) Sv129/CD-1 hybrid mice were subjected to unilateral hypoxia-ischemia and histologic damage was assessed 10 days later by two evaluators. Poly(ADP-ribose) polymerase-1 knockout produced moderate but significant (p < 0.05) protection in the total group of animals, but analysis by sex revealed that males were strongly protected (p < 0.05) in contrast to females in which there was no significant effect. Separate experiments demonstrated that PARP-1 was activated over 1-24 h in both females and males after the insult in neonatal wt mice and rats using immnocytochemistry and western blotting for poly(ADP-ribose). Brain levels of NAD+ were also significantly reduced, but the decrease of NAD+ during the early post-hypoxia-ischemia (HI) phase was only seen in males. The results indicate that hypoxia-ischemia activates Poly(ADP-ribose) polymerase-1 in the neonatal brain and that the sex of the animal strongly influences its role in the pathogenesis of brain injury.
Nyckelord
- Animals
- Animals
- Newborn
- Blotting
- Western/methods
- Brain Ischemia/genetics/metabolism/*prevention & control
- Cell Count/methods
- Female
- Functional Laterality/physiology
- Immunohistochemistry/methods
- Male
- Mice
- Mice
- Knockout
- Mortality
- NAD/metabolism
- Poly(ADP-ribose) Polymerases/*deficiency/genetics/metabolism
- Rabbits
- Sex Factors
- Time Factors
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Hagberg, Henrik, ...
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Wilson, Mary Ann
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Matsushita, Hiro ...
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Zhu, Changlian, ...
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Lange, Mary
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Gustavsson, Mali ...
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visa fler...
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Poitras, Marc F.
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Dawson, Ted. M.
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Dawson, Valina. ...
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Northington, Fra ...
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Johnston, Michae ...
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visa färre...
- Artiklar i publikationen
- J Neurochem
- Av lärosätet
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Göteborgs universitet