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Single-Dilution COVID-19 Antibody Test with Qualitative and Quantitative Readouts

Bortz, Robert H. (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States
Florez, Catalina (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Department of Chemistry and Life Science, United States Military Academy at West Point, West Point, NY, United States
Laudermilch, Ethan (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States
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Wirchnianski, Ariel S. (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States
Lasso, Gorka (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States
Malonis, Ryan J. (författare)
Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States
Georgiev, George I. (författare)
Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States
Vergnolle, Olivia (författare)
Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States
Herrera, Natalia G. (författare)
Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States
Morano, Nicholas C. (författare)
Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States
Campbell, Sean T. (författare)
Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States
Orner, Erika P. (författare)
Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States
Mengotto, Amanda (författare)
Montefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States
Dieterle, M. Eugenia (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States
Fels, J. Maximilian (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States
Haslwanter, Denise (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States
Jangra, Rohit K. (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States
Celikgil, Alev (författare)
Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States
Kimmel, Duncan (författare)
Montefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States
Lee, James H. (författare)
Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States
Mariano, Margarette C. (författare)
Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States
Nakouzi, Antonio (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States
Quiroz, Jose (författare)
Montefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States
Rivera, Johanna (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States
Szymczak, Wendy A. (författare)
Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States
Tong, Karen (författare)
Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States
Barnhill, Jason (författare)
Department of Chemistry and Life Science, United States Military Academy at West Point, West Point, NY, United States
Forsell, Mattias N. E. (författare)
Umeå universitet,Institutionen för klinisk mikrobiologi
Ahlm, Clas, 1956- (författare)
Umeå universitet,Institutionen för klinisk mikrobiologi
Stein, Daniel T. (författare)
Montefiore Medical Center, NY, Bronx, United States; Division of Endocrinology and Diabetes, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States
Pirofski, Liise-Anne (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States
Goldstein, D Yitzchak (författare)
Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States
Garforth, Scott J. (författare)
Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States
Almo, Steven C. (författare)
Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States
Daily, Johanna P. (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, NY, Bronx, United States
Prystowsky, Michael B. (författare)
Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States
Faix, James D. (författare)
Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States
Fox, Amy S. (författare)
Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States; Montefiore Medical Center, NY, Bronx, United States
Weiss, Louis M. (författare)
Montefiore Medical Center, NY, Bronx, United States; Department of Pathology, Albert Einstein College of Medicine, NY, Bronx, United States
Lai, Jonathan R. (författare)
Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States
Chandran, Kartik (författare)
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States
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 (creator_code:org_t)
American Society for Microbiology, 2021
2021
Engelska.
Ingår i: mSphere. - : American Society for Microbiology. - 2379-5042. ; 6:2
Relaterad länk:
https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
https://urn.kb.se/re...
https://doi.org/10.1...
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • The coronavirus disease 2019 (COVID-19) global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to place an immense burden on societies and health care systems. A key component of COVID-19 control efforts is serological testing to determine the community prevalence of SARS-CoV-2 exposure and quantify individual immune responses to prior SARS-CoV-2 infection or vaccination. Here, we describe a laboratory-developed antibody test that uses readily available research-grade reagents to detect SARS-CoV-2 exposure in patient blood samples with high sensitivity and specificity. We further show that this sensitive test affords the estimation of viral spike-specific IgG titers from a single sample measurement, thereby providing a simple and scalable method to measure the strength of an individual's immune response. The accuracy, adaptability, and cost-effectiveness of this test make it an excellent option for clinical deployment in the ongoing COVID-19 pandemic.IMPORTANCE Serological surveillance has become an important public health tool during the COVID-19 pandemic. Detection of protective antibodies and seroconversion after SARS-CoV-2 infection or vaccination can help guide patient care plans and public health policies. Serology tests can detect antibodies against past infections; consequently, they can help overcome the shortcomings of molecular tests, which can detect only active infections. This is important, especially when considering that many COVID-19 patients are asymptomatic. In this study, we describe an enzyme-linked immunosorbent assay (ELISA)-based qualitative and quantitative serology test developed to measure IgG and IgA antibodies against the SARS-CoV-2 spike glycoprotein. The test can be deployed using commonly available laboratory reagents and equipment and displays high specificity and sensitivity. Furthermore, we demonstrate that IgG titers in patient samples can be estimated from a single measurement, enabling the assay's use in high-throughput clinical environments.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Infectious Medicine (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Immunology in the medical area (hsv//eng)

Nyckelord

COVID-19
IgA
IgG
laboratory diagnostic test
quantitative test
SARS-CoV-2
serology
spike protein

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