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| 2. |
- Ding, Limei, et al.
(författare)
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Application of fuzzy control to a flotation process
- 1999
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Ingår i: Elsevier IFAC Publications / IFAC Proceedings series. - Kildington : Elsevier. - 1474-6670. ; 32:2, s. 6998-7003, s. 391-396
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Tidskriftsartikel (refereegranskat)abstract
- The application of fuzzy logic in the control of flotation is studied in this paper. A general description of apatite flotation process is given and a dynamic model is developed. A fuzzy logic controller is proposed for a nonlinear isolated continuous flotation process. The knowledge base of the controller is constructed on the basis of the semi-batch results from the apatite flotation experiments and available knowledge sources. The design of the controller does not need an exact process model. The simulation result shows that the fuzzy logic controller can reduce collector dosage consumption of the apatite flotation process while maintaining the phosphorous content in magnetite concentrate within an acceptable limits less than 0.025% P. Stability of the fuzzy control system is discussed
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| 3. |
- Ding, Limei, et al.
(författare)
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Fuzzy logic control of flotation process
- 1998
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Ingår i: Reglermöte '98. - Lund : Reglerteknik, Lunds tekniska högskola.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 4. |
- Ding, Limei, et al.
(författare)
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Modelling and control of a flotation process
- 1999
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Ingår i: Control and optimization in minerals, metals and materials processing. - Montreal : MetSoc. - 0919086888 ; , s. 285-298
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Konferensbidrag (refereegranskat)abstract
- A general description of a flotation process is given. The dynamic model of a MIMO nonlinear subprocess in flotation, i.e. the pulp levels in five compartments in series is developed and the model is verified with real data from a production plant. In order to reject constant disturbances five extra states are introduced and the model is modified. An exact linearization has been made for the non-linear model and a linear quadratic Gaussian controller is proposed based on the linearized model. The simulation result shows an improved performance of the pulp level control when the set points are changed or a disturbance occur. In future the controller will be tested in production
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| 5. |
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| 6. |
- Ding, W Q, et al.
(författare)
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Acute ethanol exposure attenuates expression of junD in human neuroblastoma cells
- 1998
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Ingår i: Neurochemistry International. - 0197-0186. ; 33:6, s. 525-530
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Tidskriftsartikel (refereegranskat)abstract
- This study describes the effects of acute ethanol exposure on the mRNA levels of c-jun,junB and junD in the human neuroblastoma SH-SY5Y cell line. An acute exposure to 100 mM ethanol did not influence the basal and phorbol ester-induced expression of c-jun and junB, whereas the basal mRNA level of junD was attenuated by 30%. This effect was dose- and time-dependent with maximal inhibition being detected 2 h after 100 mM ethanol treatment and the mRNA levels gradually returned towards normal afterwards. Ethanol also inhibited phorbol ester-induced expression of junD. The fact that ethanol did not influence degradation of the junD mRNA suggests that acute ethanol suppresses the transcription of the gene. These results indicate that acute ethanol exerts different effects on expression of Jun transcription factors, suggesting that as compared to c-jun and junB, the junD gene may be more sensitive to acute ethanol treatment in neuronal cells.
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| 7. |
- Ding, W Q, et al.
(författare)
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Effects of ethanol on muscarinic receptor-stimulated c-fos expression in human neuroblastoma cells
- 1997
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Ingår i: Brain Research. Molecular Brain Research. - 0169-328X. ; 46:1-2, s. 77-84
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Tidskriftsartikel (refereegranskat)abstract
- The effect of ethanol exposure on muscarinic receptor-stimulated expression of c-fos was investigated in SH-SY5Y cells. Four days of ethanol exposure enhanced carbachol-stimulated c-fos mRNA expression, analyzed with Northern blot, and Fos/AP-1 binding activity, measured with gel mobility super shift assay. Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Acute exposure to ethanol caused a suppression of both carbachol- and phorbol ester-stimulated c-fos expression. These results demonstrate that muscarinic receptor-stimulated gene expression is sensitive to both acute and long-term ethanol exposure.
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| 8. |
- Ding, Wei-Qun
(författare)
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Effects of ethanol on the expression of immediate-early genes in nerve cells
- 1998
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Immediate-early genes of the fos and jun families encode proteins that dimerize to form a complex of activator protein 1 and regulate the expression of a number of genes in the nervous system. Ethanol has previously been shown to induce alterations in expression of fos and jun genes in the brain. The present study examined the effects of ethanol on the expression of c-fos, fosB, c-jun, junB and junD genes in human neuroblastoma SH-SY5Y cells. Prior to the investigation of ethanol effects, the basal and agonist-stimulated expression of these genes were characterized. A constitutive expression of c-jun and junD was observed in unstimulated cells, whereas transcripts of c-fos, fosB and junB could not be detected. Stimulation with carbachol and TPA induced expression of all these genes but the expression patterns differed among individual genes. The expression of c-fos, fosB and junB induced by carbachol was primarily mediated through protein kinase C, whereas the carbachol-stimulated expression of c-jun and junD was mainly regulated via calcium/calmodulin-dependent kinase II. Long-term ethanol exposure increased the expression of c-jun and junD and potentiated the carbachol-stimulated expression of c-fos, fosB and junB. On the other hand, acute ethanol selectively attenuated the basal and phorbol ester-stimulated expression of junD, leaving the expression of c-jun and junB unaffected. Thus, junD seems to be sensitive to both acute and long-term ethanol exposure. The potentiation of carbachol-stimulated c-fos, fosB and junB expression after long-term ethanol exposure was primarily mediated via M1 receptors and protein kinase C. Neither long-term nor acute ethanol exposure influenced the degradation of the junD mRNA, which indicates that it is the transcription of the gene which is affected by ethanol. These results support the view that expression of fos and jun genes is involved in the ethanol-induced changes in the function of nerve cells.
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| 9. |
- Ding, Wei-Qun, et al.
(författare)
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Ethanol exposure increases expression of c-jun and junD in human neuroblastoma cells
- 1996
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Ingår i: NeuroReport. - 1473-558X. ; 7:13, s. 2191-2195
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to investigate the effect of ethanol exposure on the expression of fos and jun genes. Exposure of human neuroblastoma SH-SY5Y cells to ethanol for 2-4 days caused a dose-dependent increase in c-jun and junD mRNA levels, whereas mRNAs for c-fos, fosB and junB were not detectable in control or ethanol-treated cells. Four days of ethanol exposure also enhanced the AP-1 binding activity. Experiments with actinomycin D demonstrated that ethanol did not influence the degradation of c-jun and junD mRNAs. These results demonstrate that long-term exposure to ethanol increases c-jun and junD expression. This effect may be one of the mechanisms through which ethanol influences the gene regulatory system in neuronal cells.
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| 10. |
- Ding, Wei-Qun, et al.
(författare)
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Ethanol exposure potentiates fosB and junB expression induced by muscarinic receptor stimulation in neuroblastoma SH-SY5Y cells
- 1998
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Ingår i: Alcoholism: Clinical and Experimental Research. - : Wiley. - 0145-6008 .- 1530-0277. ; 22:1, s. 225-230
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Tidskriftsartikel (refereegranskat)abstract
- Muscarinic receptor stimulation and activation of protein kinase C cause an increase in fosB and junB transcripts in human neuroblastoma SH-SY5Y cells. In this study, the effect of long-term ethanol exposure on these events was investigated. Carbachol-stimulated fosB and junB expression was elevated in ethanol-exposed cells compared with control cells. The potentiation was time- and dose-dependent on ethanol. Preincubation with muscarinic antagonists or protein kinase C inhibitor demonstrated that the carbachol-stimulated increase in fosB and junB mRNA levels was primarily mediated via M1 receptors and dependent on the activity of protein kinase C in both control and ethanol-exposed cells. Long-term ethanol exposure did not influence the expression of fosB and junB induced by activation of protein kinase C with phorbol ester. These results demonstrate that the muscarinic receptor-stimulated fosB and junB expression is sensitive to ethanol exposure in SH-SY5Y cells, suggesting that these genes participate in the regulation of neuronal function in response to chronic ethanol treatment.
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