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- Brix-Christensen, V., et al.
(författare)
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Plasma cytokines do not reflect expression of pro- and anti-inflammatory cytokine mRNA at organ level after cardiopulmonary bypass in neonatal pigs.
- 2003
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Ingår i: Acta Anaesthesiologica Scandinavica. - : Wiley. - 0001-5172. ; 47:5, s. 525-531
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Tidskriftsartikel (refereegranskat)abstract
- Background: Plasma concentrations of inflammatory markers are increased in response to the trauma of cardiac surgery and cardiopulmonary bypass (CPB). It is, however, unknown whether the plasma cytokine levels and cytokine mRNA expression at organ level reflect each other. Methods: Twenty-six piglets (17–19 days) were allocated to the sham-group (sternotomy only, n = 13) or to the CPB-group (sternotomy, 120 min CPB procedure with 60-min aortic cross-clamp, n = 13). The pigs were observed for 0.5 h or 4 h post-CPB. Plasma levels of IL-1β, IL-6, IL-8 and IL-10 and mRNA expression of TNF-α, IL-1β, IL-6, IL-8, IL-10 and iNOS in organs were registered with concomitant changes in oxygenation index (OI) and expiratory nitric oxide (NO). Results: In pigs killed 0.5 h post-CPB there was a significant increase in IL-10 mRNA in the lungs and kidneys compared with the sham-group. IL-1β mRNA was detectable in the kidneys and lungs of the CPB-pigs, while IL-6 mRNA was up regulated only in lungs. In pigs killed 4 h post-CPB a significantly higher IL-6 mRNA was found in heart tissue and a lower IL-10 mRNA was found in lungs of CPB pigs compared with the sham-group. There was a concomitant significant increase in OI and increased plasma IL-8 and IL-10 concentrations in the CPB-pigs compared with the sham-pigs. Conclusion: The cytokine mRNA expression pattern was very different for the pigs killed already 0.5 h after the CPB procedure compared with the pigs killed 4 h post-CPB. The plasma cytokine levels poorly reflected mRNA expression of the pro- and anti-inflammatory cytokines.
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| 2. |
- Dreyer, C, et al.
(författare)
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Raman scattering at 532 and 355 nm in atmospheric pressure propane/air flames, with and without liquid fuels
- 2004
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Ingår i: Applied Physics B. - : Springer Science and Business Media LLC. - 0946-2171 .- 1432-0649. ; 79:1, s. 121-130
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Tidskriftsartikel (refereegranskat)abstract
- We discuss an experimental investigation of Raman scattering spectroscopy in the vicinity of a simulated reacting liquid fuel spray. Spray flames are known to produce interference from droplet elastic scattering, laser-induced fluorescence, and laser-induced incandescence of soot. The goal in this work is to evaluate various experimental approaches to this challenging problem. We evaluate Raman scattering from both 532 and 355 nm beams (second and third harmonics of Nd : YAG), at various polarization orientations and over various lifetimes. We discuss tradeoffs between the various approaches and identify operating regimes in which such Raman measurements are possible. Perhaps the most compelling finding of this work is that the third harmonic of Nd : YAG holds more promise for measurement in high-pressure, combusting spray environments than has been previously thought.
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