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Ultra-fast proteomi...
Ultra-fast proteomics with Scanning SWATH
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- Messner, Christoph B. (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin,The Francis Crick Institute
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- Demichev, Vadim (author)
- The Francis Crick Institute,University Of Cambridge,Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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Bloomfield, Nic (author)
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- Yu, Jason S.L. (author)
- The Francis Crick Institute
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- White, Matthew (author)
- The Francis Crick Institute
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- Kreidl, Marco (author)
- The Francis Crick Institute
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- Egger, Anna Sophia (author)
- The Francis Crick Institute
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- Freiwald, Anja (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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Ivosev, Gordana (author)
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Wasim, Fras (author)
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- Zelezniak, Aleksej, 1984 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,The Francis Crick Institute
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- Jürgens, Linda (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Suttorp, Norbert (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Sander, Leif Erik (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Kurth, Florian (author)
- Universitatsklinikum Hamburg-Eppendorf und Medizinische Fakultat,Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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- Lilley, Kathryn S. (author)
- University Of Cambridge
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- Mülleder, Michael (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin
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Tate, Stephen (author)
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- Ralser, M. (author)
- Charité Universitätsmedizin Berlin,Charité University Medicine Berlin,The Francis Crick Institute
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(creator_code:org_t)
- 2021-03-25
- 2021
- English.
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In: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1087-0156 .- 1546-1696. ; 39:7, s. 846-854
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https://www.nature.c...
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https://doi.org/10.1...
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Abstract
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- Accurate quantification of the proteome remains challenging for large sample series and longitudinal experiments. We report a data-independent acquisition method, Scanning SWATH, that accelerates mass spectrometric (MS) duty cycles, yielding quantitative proteomes in combination with short gradients and high-flow (800 µl min ) chromatography. Exploiting a continuous movement of the precursor isolation window to assign precursor masses to tandem mass spectrometry (MS/MS) fragment traces, Scanning SWATH increases precursor identifications by ~70% compared to conventional data-independent acquisition (DIA) methods on 0.5–5-min chromatographic gradients. We demonstrate the application of ultra-fast proteomics in drug mode-of-action screening and plasma proteomics. Scanning SWATH proteomes capture the mode of action of fungistatic azoles and statins. Moreover, we confirm 43 and identify 11 new plasma proteome biomarkers of COVID-19 severity, advancing patient classification and biomarker discovery. Thus, our results demonstrate a substantial acceleration and increased depth in fast proteomic experiments that facilitate proteomic drug screens and clinical studies. –1
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk bildbehandling (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Image Processing (hsv//eng)
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- By the author/editor
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Messner, Christo ...
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Demichev, Vadim
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Bloomfield, Nic
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Yu, Jason S.L.
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White, Matthew
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Kreidl, Marco
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show more...
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Egger, Anna Soph ...
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Freiwald, Anja
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Ivosev, Gordana
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Wasim, Fras
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Zelezniak, Aleks ...
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Jürgens, Linda
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Suttorp, Norbert
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Sander, Leif Eri ...
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Kurth, Florian
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Lilley, Kathryn ...
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Mülleder, Michae ...
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Tate, Stephen
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Ralser, M.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Pharmaceutical S ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Analytical Chemi ...
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Medical Engineer ...
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and Medical Image Pr ...
- Articles in the publication
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Nature Biotechno ...
- By the university
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Chalmers University of Technology