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- Bilgin, Neş'e, et al.
(författare)
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Mutations in ribosomal proteins L7/L12 perturb EF-G and EF-Tu functons
- 1988
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Ingår i: Biochimie. - : Elsevier BV. - 0300-9084 .- 1638-6183. ; 70:5, s. 611-618
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Tidskriftsartikel (refereegranskat)abstract
- In vitro cycling rates of E. coli ribosomes and of elongation factors EF-Tu and EF-G have been obtained and these are compatible with translation rates in vivo. We show that the rate of translocation is faster than 50 s-1 and therefore that the EF-G function is not a rate limiting step in protein synthesis. The in vivo phenotype of some L7/L12 mutants could be accounted for by perturbed EF-Tu as well as EF-G functions. The S12 mutants that we studied were, in contrast, only perturbed in their EF-Tu function, while their EF-G interaction was not impaired in relation to wild type ribosomes.
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