| 1. |
- Augustsson, Per, et al.
(författare)
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Decomplexing biofluids using microchip based acoustophoresis
- 2009
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Ingår i: Lab on a Chip. - 1473-0189. ; 9:6, s. 810-818
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Tidskriftsartikel (refereegranskat)abstract
- Highly efficient washing and extraction of microbeads to decomplex analytes ranging from small peptides to large viruses was realised in a microscaled continuous flow format. The bead washing principle reported herein is based on acoustophoresis, i.e. the primary acoustic radiation force in an ultrasonic standing wave and laminar flow properties are utilised to translate bioanalytes trapped on functionalised microbeads from one carrier fluid to another. The carry-over of non-specific material ranges from 1 to 50 ppm relative to input levels depending on application, making acoustophoresis suitable for extraction of rare species from complex environments. Selective extraction of a phosphopeptide relative to its unphosphorylated counterpart is demonstrated using metal oxide affinity capture (MOAC) beads and MALDI-TOF MS readout. Acoustophoresis of microbeads activated with specific binders could be used to capture phage viral particles. The efficiency of the acoustophoretic washing principle was demonstrated by an unspecific phage cross contamination level of only 10(-6) of that in the input bead/phage mixture. The continuous flow format makes acoustophoretic washing flexible regarding sample volume and also allows for easy integration into a sequence of particle handling and analytical unit operations.
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| 4. |
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| 5. |
- Ekström, Simon, 1966-
(författare)
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Hockeybockarna, skandalen och moralen.
- 2005
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Ingår i: Tankar från baslinjen.. - Eslöv : Brutus Östlings Bokförlag Symposion, Stockholm. ; , s. 119-136
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 6. |
- Ekström, Simon, et al.
(författare)
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Integrated selective enrichment target (ISET) - A generic microfabricated sample preparation device
- 2005
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Ingår i: Micro Total Analysis Systems 2004, Vol 2. - 0260-6291. - 9780854048960 ; :297, s. 548-550
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Konferensbidrag (refereegranskat)abstract
- A combined sample preparation and sample presentation device, Integrated Selective Enrichment Target (ISET), consisting of an array with 96 perforated nanovials is described. Each perforated nanovial can be filled with soild-phase extraction media for purification and concentration of biomolecules prior to mass spectrometry. The ISET platform provides an efficient, economic and generic sample treatment process, allowing for rapid in-parallel handling of minute sample volumes, with a minimum of sample transfers.
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| 7. |
- Ekström, Simon
(författare)
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Jacob Wenzer : Resonanser
- 2007
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Ingår i: RIG: Kulturhistorisk tidskrift.. - 0035-5267. ; :3
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Recension (övrigt vetenskapligt/konstnärligt)
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| 8. |
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| 9. |
- Ekström, Simon, et al.
(författare)
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Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target
- 2006
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 5:5, s. 1071-1081
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Tidskriftsartikel (refereegranskat)abstract
- A microfabricated proteomic sample preparation and sample presentation device, Integrated Selective Enrichment Target, (ISET), comprising an array of 96 perforated nanovials is described. Each perforated nanovial can be filled with solid-phase extraction media for purification and concentration of peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI IVIS). The validity of the ISET sample preparation is shown by analysis of low nM-pM standard samples, as well as biological samples. The ISET solid-phase extraction sample preparation was compared to ZipTip and MassPREP PROtarget sample preparation, demonstrating a superior performance with respect to number of detected peptides and signal intensity of detected peptides.
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| 10. |
- Ekström, Simon
(författare)
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On chip integrated sample preparation for proteomics
- 2005
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The aim of this thesis project has been to create miniaturized analytical tools that improve sample preparation for the very rapidly growing field of proteomics research. The presented miniaturized analytical tools provide novel and generic analytical solutions, which are highly adaptable for analysis of various low abundant biomolecules. The adaptability and generality of the analytical technology is very important since there are a great diversity both in the analysis methods used and the biomolecules themselves. This technology is achieved through the use of silicon micromachning. The thesis presents microstructure developments both in the form of improvements of existing technology and new hardware in the form of microstructures for sample preparation purposes in proteomics. This is achieved by integrated on-chip sample preparation enabling enrichment and sensitive analysis by mass spectrometry. A sample preparation platform (spot-on-a-chip) consisting of a microdispenser and nanovial arrays enables efficient protein enrichment and sensitive microspot sample preparation for Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis is presented. Technology for improved enzymatic digestion and highly efficient solid-phase microextraction is also demonstrated.
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