| 1. |
- Adler, Belinda, et al.
(författare)
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Miniaturized and Automated High-Throughput Verification of Proteins in the ISET Platform with MALDI MS
- 2012
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 84:20, s. 8663-8669
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Tidskriftsartikel (refereegranskat)abstract
- A major bottleneck in high-throughput protein production is the validation step, which is why parallel and automated sample processing methods are highly desirable. Also, a miniaturized sample preparation format is preferred, as the reduction of reagent volumes significantly decreases the analysis cost per sample. We have developed an automated and miniaturized protein sequence verification protocol for recombinant proteins utilizing peptide mass fingerprinting and MS/MS analysis. The integrated selective enrichment target (ISET) platform, previously developed in our group, with its dual functionality, being both a sample preparation platform and a MALDI target plate, is employed. All steps including immobilized metal ion affinity chromatography of protein on cobalt-loaded beads, tryptic digestion, and MALDI MS analysis are performed in an array format, without any sample transfers, on the same ISET chip. The automated configuration reduced the sample preparation time significantly. Starting with crude lysate, a full plate of 48 purified, digested samples prepared for MALDI-MS can be generated in 4 h, with only 30 min of operator involvement. This paper demonstrates the utility of the method by parallel analysis of 45 His-tagged human recombinant proteins.
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| 2. |
- Adler, Belinda, et al.
(författare)
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Optimizing nanovial outlet designs for improved solid-phase extraction in the integrated selective enrichment target-ISET.
- 2012
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Ingår i: Electrophoresis. - : Wiley. - 0173-0835. ; 33:21, s. 3143-3150
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Tidskriftsartikel (refereegranskat)abstract
- The integrated selective enrichment target is a microfluidic platform for SPE sample preparation with integrated nanocolumns, which simultaneously offers direct MALDI MS read-out. Here, we present a study on the importance of different nanocolumn outlet hole geometries and hole areas in relation to MS signal intensity and reproducibility. A design solution that provides the flow characteristics required for robust sample preparation using automated liquid handling is reported.
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| 3. |
- Ahmad Tajudin, Asilah, et al.
(författare)
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MALDI-target integrated platform for affinity-captured protein digestion.
- 2014
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Ingår i: Analytica Chimica Acta. - : Elsevier BV. - 1873-4324 .- 0003-2670. ; 807:Jan 7, s. 1-8
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Tidskriftsartikel (refereegranskat)abstract
- To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40fmol to 1pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented.
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| 4. |
- Augustsson, Per, et al.
(författare)
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Acoustophoretic microfluidic chip for sequential elution of surface bound molecules from beads or cells
- 2012
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Ingår i: Biomicrofluidics. - : AIP Publishing. - 1932-1058. ; 6:3
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Tidskriftsartikel (refereegranskat)abstract
- An acoustophoresis-based microfluidic flow-chip is presented as a novel platform to facilitate analysis of proteins and peptides loosely bound to the surface of beads or cells. The chip allows for direct removal of the background surrounding the beads or cells, followed by sequential treatment and collection of a sequence of up to five different buffer conditions. During this treatment, the beads/cells are retained in a single flow by acoustic radiation force. Eluted peptides are collected from the outlets and subsequently purified by miniaturized solid-phase extraction and analyzed with matrix assisted laser desorption mass spectrometry. Fundamental parameters such as the system fluidics and dispersion are presented. The device was successfully applied for wash and sequential elution of peptides bound to the surface of microbeads and human spermatozoa, respectively. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4749289]
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| 5. |
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| 6. |
- Ekström, Simon, 1966-
(författare)
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Begrepp i bur : Om kulturbruk och kulturbråk
- 2010
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Ingår i: Kulturella perspektiv - Svensk etnologisk tidskrift. - 1102-7908. ; 19:3, s. 5-17
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Tidskriftsartikel (refereegranskat)abstract
- The Swedish debate concerning honour killings has largely revolved around the concept of “culture” – or more specifically, it has revolved around the ways in which different writers have criticized some other debaters for misusing the concept. Hence, this debate sheds light on how the concept of culture has come to occupy a position in something one might call a linguistic quarantine. Cultural explanations are often considered an issue which must be handled with great caution in order not to explode into cultural racism, or to an undesired exaggeration of cultural difference. Moreover, the most serious objections against the con- cept of culture seem to occur in the very field which the concept has traditionally been regarded as most suitable for. Recent controversies around phenomena such as honour killings, structural dis- crimination and cultural racism reveals that it first and foremost is in relation to the “exotic other” that cultural explanations nowadays are becoming questioned. On the other hand, few debaters and researchers are ready to seriously do away with a concept they continue to understand as applicable for their own analyses. The aim of this article is, therefore, to highlight some of the critical arguments in this debate, so as to discuss the ambivalences that distinguish contemporary public disputes about culture and cultural explanations. keywords: the concept of culture, honour killing, linguistic quarantine, culture racism.
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| 7. |
- Ekström, Simon, 1966-
(författare)
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Den flyktiga fjärilen
- 2014
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Ingår i: Svenska sjömanstatueringar. - Stockholm : Medströms Bokförlag. - 9789173291170 ; , s. 129-138
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 8. |
- Ekström, Simon, 1966-
(författare)
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Följa hummer
- 2013
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Ingår i: RIG. - 0035-5267 .- 2002-3863. ; 96:2, s. 65-79
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Tidskriftsartikel (refereegranskat)abstract
- Following the LobsterIn this article, lobsters serve as an analytical trace element for a further discussion about the mutual relation between materiality and meaning. Through keystrokes in different historical and contemporary contexts, the first part of the text presents a number of examples of what a lobster could be – and what it could also be expected to do. The second part discusses the act of following as a culture-analytical method, and indicates the benefits that concepts like frequency, density and population can have.Asking oneself what a lobster could be underlines that lobsters have more than one meaning. In other words, it is a way of helping us to see how the meaning we ascribe to a lobster is dependent on the kind of company it keeps. Lobsters seldom appear alone. On the contrary, they are almost always surrounded by other things, at the same time as they are objects for highly varying practices. For someone whose task it is to actively look for lobsters, i.e. what in the article is called following the lobster, this is especially clear. When lobsters are moved from one context to another, the setting is also changed. The earlier network of objects, people and physical environments that surrounded the lobsters changes shape. For lobsters, every new setting also means a new network.In this way, the following of lobsters illustrates how translations and meaning-making should be understood as both material and cultural phenomenon. In the analysis, the object is always one and the same – lobsters – while the setting and the network can change several times by simply twisting the lens. All this can be regarded as a kaleidoscopic cultural analysis, where the ambition is to discover something new about the lobster by searching for it in expected as well as unexpected places. At the same time, the intention is also to generate new knowledge about the contexts and activities that are actualised by the presence of the lobsters. This is why the article’s recurring question – which is posed in the presence of every specific lobster population – is, what is the work that the lobsters are expected to do, right there and then?
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| 9. |
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| 10. |
- Ekström, Simon, 1966-
(författare)
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Pinuppan : påklätt, avklätt, naket
- 2014
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Ingår i: Svenska sjömanstatueringar. - Stockholm : Medströms Bokförlag. - 9789173291170 ; , s. 139-147
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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