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Träfflista för sökning "WFRF:(Engelmark Cassimjee Karim) srt2:(2006-2009)"

Sökning: WFRF:(Engelmark Cassimjee Karim) > (2006-2009)

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  • Cassimjee, Karim Engelmark, et al. (författare)
  • Silica-immobilized His(6)-tagged enzyme : Alanine racemase in hydrophobic solvent
  • 2008
  • Ingår i: Biotechnology and Bioengineering. - : Wiley. - 0006-3592 .- 1097-0290. ; 99:3, s. 712-716
  • Tidskriftsartikel (refereegranskat)abstract
    • A new immobilization method for enzymes is presented to facilitate synthetic applications in aqueous as well as organic media. The enzyme Alanine racemase (AlaR) from Geobacillus stearothermophilus was cloned, overexpressed and then immobilized on a silica-coated thin-layer chromatography plate to create an enzyme surface. The enzyme, fused to a His(6)-tag at its N-terminal, was tethered to the chemically modified silica-coated TLC plate through cobalt ions. The immobilized enzyme showed unaltered kinetic parameters in small-scale stirred reactions and retained its activity after rinsing, drying, freezing or immersion in n-hexane. This practical method is a first step towards a general immobilization method for synthesis applications with any enzyme suitable for His(6)-tagging.
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  • Hauer, Bernhard, et al. (författare)
  • Process for producing polyamines
  • 2009
  • Patent (populärvet., debatt m.m.)abstract
    • The present invention relates to a process for the production of a polyamine involving the use of enzymes; in particular to a process performed in aqueous environment; to the polyamines produced by said method; as well as the use of said polyamines for manufacturing paper, for immobilizing enzymes, or for preparing pharmaceutical or cosmetical compositions. The invention also relates to a novel method for in situ regeneration of cofactors NAD(P)+.
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  • Resultat 1-8 av 8

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