| 1. |
- Englund, Ulrica, et al.
(författare)
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The use of a recombinant lentiviral vector for ex vivo gene transfer into the rat CNS
- 2000
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Ingår i: NeuroReport. - : Lippincott Williams & Wilkins. - 0959-4965 .- 1473-558X. ; 11:18, s. 3973-3977
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Tidskriftsartikel (refereegranskat)abstract
- A major obstacle in ex vivo gene transfer has been the loss of transgene expression soon after implantation of the grafted transduced cells. Recently, a lentiviral vector system has been developed which has proven to express high levels of transgenes in vivo after direct injection into the tissue. In this study, we have investigated the use of such a vector for ex vivo gene transfer to the brain. A number of neural cell types were found to be permissive to transduction by the lentiviral vector in vitro and a majority of them expressed the transgene after transplantation to the rat brain. Transgene expression was detected up to 8 weeks post-grafting. These findings suggest that recombinant lentiviral vectors may be used for further development of ex vivo gene therapy protocols to the CNS.
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| 2. |
- Ericson, Cecilia, et al.
(författare)
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Ex vivo and in vitro studies of transgene expression in rat astrocytes transduced with lentiviral vectors.
- 2002
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Ingår i: Experimental Neurology. - : Elsevier BV. - 0014-4886. ; 173:1, s. 22-30
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Tidskriftsartikel (refereegranskat)abstract
- Implantation of cells genetically modified to express therapeutic genes into the brain has been proposed as a potential treatment for neurodegenerative diseases. In the current study embryonic rat-derived astrocytes were cultured and transduced with a lentiviral vector expressing the reporter gene green fluorescent protein (GFP) and subsequently grafted into the adult rat brain. The proportion of GFP expressing cells was stable, albeit small (1%), at all survival times, up to 6 weeks, the longest time point studied. In parallel in vitro studies, the astrocytes were lentivirally transduced to express either one of the two isoforms of glutamate decarboxylase (GAD(65) or GAD(67)) or glial cell line-derived neurotrophic factor (GDNF). When transducing 293T cells with the two GAD vectors, released GABA could be measured using high-performance liquid chromatography. Further studies of rat astrocytes transduced with the same vectors resulted in a level of GAD activity about 10 times higher than the activity of an intact rat striatum. One hundred thousand astrocytes transduced with LV-GDNF released approximately 27 ng of GDNF per hour. Thus, taken together, our observations provide support for the use of rat astrocytes in ex vivo gene transfer of these proteins in animal models of CNS disorders, e.g., Parkinson's disease or epilepsy.
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| 3. |
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| 4. |
- Zhang, Qiuxia, 1960, et al.
(författare)
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Blood flow in the tibialis anterior muscle by photoplethysmography during foot-transmitted vibration.
- 2003
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Ingår i: European journal of applied physiology. - : Springer Science and Business Media LLC. - 1439-6319 .- 1439-6327. ; 90:5-6, s. 464-9
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Tidskriftsartikel (refereegranskat)abstract
- Photoplethysmography (PPG) was used to detect changes in the anterior tibial muscle blood flow (MBF) during foot-transmitted vibration in six healthy subjects. A filter was developed for reducing the vibration-induced artefacts in the PPG signal. The vibration applied was random with constant acceleration power density in the range 5-2000 Hz with an acceleration of 16-46 m s(-2) (rms). The application of the filter technique showed a dramatic reduction in the vibration-induced artefacts and distortion of the PPG signal was negligible. The mean ratio and correlation coefficient of MBF originating from filtered and non-filtered PPG signals was [mean (SD)] 0.89 (0.04) and 0.99, respectively. This made it possible to detect a relative increase in MBF of 20% during an acute vibration exposure. The results suggest that the use of the filter enables MBF to be measured by PPG during vibration, thus extending the range of applications of the PPG technique to include ergonomic conditions.
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