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- Engstrand, Mats, et al.
(författare)
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Characterization of CMVpp65-specific CD8+ T lymphocytes using MHC tetramers in kidney transplant patients and healthy participants
- 2000
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Ingår i: Transplantation. - : Ovid Technologies (Wolters Kluwer Health). - 0041-1337 .- 1534-6080. ; 69:11, s. 2243-2250
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Cytomegalovirus (CMV) is a ubiquitous herpesvirus that infects 50-90% of individuals in different populations. After primary infection, the virus persists latently in myeloid cells under the control of specific T-cells. Reactivation of CMV infection may cause lethal organ dysfunction and is frequently seen in immunosuppressed individuals. CD8+ cytotoxic T-cells (CTL) have a primary role in suppressing CMV reactivation, and the dominating CTL response is directed against pp65. METHODS: MHC tetramers, that is, complexes between HLA class I (or class II) molecules and antigenic peptides conjugated to fluorochromes allow the direct visualization of antigen-specific receptor-carrying T-cells using flow cytometry. We constructed a novel MHC tetramer for identification of CMVpp65-specific CD8+ T-cells using HLA-A2 molecules folded with the immunodominant NLVPMVATV peptide. RESULTS: The A2/pp65 tetramer specifically stained CMV-directed T-cell lines, and sorted cells showed CMV-specific cytotoxicity. High proportions (0.1-9%) of the CD8+ T-cells were A2/pp65 tetramer+ in healthy HLA-A2+ CMV carriers and in immunosuppressed kidney transplant patients with latent infection. Patients with reactivated CMV infection exhibited up to 15% A2/pp65 tetramer+ cells, which seemed to correlate with CMV load over time. A2/pp65 tetramer+ cells expressed T-cell activation markers. CONCLUSIONS: The construction of a novel A2/pp65 MHC tetramer enabled the design of a rapid and precise flow cytometric method allowing quantitative and qualitative analysis of CMV-specific T-cells. The number of A2/pp65 tetramer binding CTLs in blood may prove to be clinically relevant in assessing the immune response to CMV.
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| 4. |
- Annerbrink, Kristina, 1974, et al.
(författare)
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Serotonin depletion increases respiratory variability in freely moving rats: implications for panic disorder.
- 2003
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Ingår i: The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP). - 1469-5111. ; 6:1, s. 51-6
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Tidskriftsartikel (refereegranskat)abstract
- To elucidate if serotonergic transmission affects respiratory variability, a parameter consistently found increased in patients with panic disorder, we studied the effect of a serotonin synthesis inhibitor, para-chlorophenylalanine (PCPA), on respiratory variability at baseline and during CO2-induced hyperventilation in awake and unrestrained rats. Forty male Wistar rats were given intraperitoneal injections of PCPA (300 mg/kg) or saline 72, 48 and 24 h before registration of respiration in a plethysmograph allowing the animals to move freely. PCPA-treated rats displayed significantly higher tidal volume variability and minute volume variability, both at baseline and during CO2 exposure, compared to controls. The results support the notion that serotonin dysfunction may contribute to the enhanced respiratory variability observed in patients with panic disorder.
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| 6. |
- Axelsson, Charlotte, 1970, et al.
(författare)
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SpringFlow: A Digital Spring-sign
- 2002
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Ingår i: Proceedings of the second Nordic conference on Human-computer interaction , Aarhus, Denmark, Jun. 2005.. - 1581136161 ; , s. 297-298
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Konferensbidrag (refereegranskat)abstract
- We present SpringFlow, a digital Spring-sign, which, from February to May, changes its characteristics to indicate how far gone spring is. With the aid of our Spring-sign, you navigate through time just like you would with a calendar. Its construction resembles a hollow ball, while the appearance of it depends on the users interactions. By tilting it, changes in sound, light, heat and cold will be produced. Based upon prior work in ubiquitous computing, SpringFlow incorporates old techniques to create something new. This paper describes the components, interaction, implementation, conceptual approach, but most of all the aesthetics
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| 8. |
- Bylund, Markus, et al.
(författare)
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Exploring the importance of context parameters for service use in everyday situations
- 2004. - 1
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The work described herein focus on the impact of different context factors in short-term planning situations. We have investigated which services have been used to solve different tasks. The method used in the study was based on scenario descriptions. The participants reported how they would have acted in different situations and they also rated the importance of different context factors in different situations. Analyses were made that revealed relationships between context factors and services used by the participants of the study. A qualitative analysis was also conducted, with the aim to capture context factors not covered in the study design.
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