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Träfflista för sökning "WFRF:(Ersson Bo) srt2:(2005-2009)"

Sökning: WFRF:(Ersson Bo) > (2005-2009)

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1.
  • Xue, Bo, et al. (författare)
  • Chromatographic and fluorometric study of interactions between thiophilic and hydrophobic ligands and tryptophan peptide homologues
  • 2006
  • Ingår i: Journal of Chromatography A. ; 1107:1-2, s. 46-51
  • Tidskriftsartikel (refereegranskat)abstract
    • The interactions of tryptophan and its peptide homologues with thiophilic ligands were studied in terms of their chromatographic retention and steady-state fluorescence under various conditions, and compared with non-polar structures typically regarded as pure hydrophobic ligands. The experimental results show that both non-polar and polar interactions are involved in what has been termed “thiophilic adsorption chromatography”.
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2.
  • Arnell, Robert, et al. (författare)
  • Biotechnological Approach to the Synthesis of 9α-Hydroxylated Steroids
  • 2007
  • Ingår i: Preparative Biochemistry & Biotechnology. - : Informa UK Limited. - 1082-6068 .- 1532-2297. ; 37:4, s. 309-321
  • Tidskriftsartikel (refereegranskat)abstract
    • The steroid 9α-hydroxylase gene has been cloned from Mycobacterium smegmatis into Escherichia coli BL21. Progesterone added to bioreactors was subjected to in vivo transformation into 9α-hydroxyprogesterone. In 7 days, 43.6 mg9α-hydroxyprogesterone was formed from 53.8 mg/L progesterone. The enzyme also has shown evidence of processing 4-androstene-3,17-dione in vivo. An extensive analytical method development, including LLE, HPLC-DAD, MS, andNMR was performed to verify the product and to enable a quantitative analysis. Protocols for analytical and preparative separation have been developed, using binaphtol as internal standard. Both the growth pattern and the bioconversion ratewere unaffected by the presence of binaphtol in the bioreactor. The enzyme was purified by immobilised metal affinity and ion exchange chromatography, resulting in low in vitro activity.
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  • Resultat 1-2 av 2
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refereegranskat (2)
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Caldwell, Karin (2)
Ersson, Bo (2)
Fornstedt, Torgny (1)
Xue, Bo (1)
Arnell, Robert (1)
Johannisson, Rickard (1)
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Lindholm, Johan (1)
Ballagi, Andras (1)
Porath, Jerker (1)
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