| 1. |
- Brockmöller, Scarlet F., et al.
(författare)
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Integration of metabolomics and expression of glycerol-3-phosphate acyltransferase (GPAM) in breast cancer-link to patient survival, hormone receptor status, and metabolic profiling
- 2012
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 11:2, s. 850-60
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Tidskriftsartikel (refereegranskat)abstract
- Changes in lipid metabolism are an important but not well-characterized hallmark of cancer. On the basis of our recent findings of lipidomic changes in breast cancer, we investigated glycerol-3-phosphate acyltransferase (GPAM), a key enzyme in the lipid biosynthesis of triacylglycerols and phospholipids. GPAM protein expression was evaluated and linked to metabolomic and lipidomic profiles in a cohort of human breast carcinomas. In addition, GPAM mRNA expression was analyzed using the GeneSapiens in silico transcriptiomics database. High cytoplasmic GPAM expression was associated with hormone receptor negative status (p = 0.013). On the protein (p = 0.048) and mRNA (p = 0.001) levels, increased GPAM expression was associated with a better overall survival. Metabolomic analysis by GC-MS showed that sn-glycerol-3-phosphate, the substrate of GPAM, was elevated in breast cancer compared to normal breast tissue. LC-MS based lipidomic analysis identified significantly higher levels of phospholipids, especially phosphatidylcholines in GPAM protein positive tumors. In conclusion, our results suggest that GPAM is expressed in human breast cancer with associated changes in the cellular metabolism, in particular an increased synthesis of phospholipids, the major structural component of cellular membranes.
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| 2. |
- Mirzaei, Maryam, 1982, et al.
(författare)
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Large Eddy Simulation of the flow and heat transfer in a half-corrugated channel with various wave amplitudes
- 2014
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Ingår i: International Journal of Heat and Mass Transfer. - : Elsevier BV. - 0017-9310. ; 76, s. 432-446
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Tidskriftsartikel (refereegranskat)abstract
- Large Eddy Simulation (LES) of turbulent flow and convective heattransfer over a half-corrugated channel is presented in this paper. Simulationsare performed for various ranges of the normalized wave amplitudes,AM = 0 − 0.15 (the ratio of wave height to wave length). The Reynoldsnumber based on the bulk velocity is chosen as Reb = 10 000 and thePrandtl number is Pr=0.71. A comparison between the DNS and LESresults of a plane channel (AM = 0) at Re = 395 is also performed.The obtained results indicate that the region of recirculating flow dependsstrongly on the wave amplitude. This study shows that the Nusseltnumber (Nu) increases by increasing the wave amplitude until a specificvalue then it remains approximately constant. The thermal performanceparameter (JF) is used as a measure for the heat transfer enhancementrelative to the pressure drop and it is found that the maximum values ofNu and JF appear at AM = 0.1, which hence correspond to the optimumvalue of the wave amplitude.
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| 3. |
- Mirzaei, Maryam, 1982, et al.
(författare)
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The effect of corrugation on heat transfer and pressure drop in channel flow with different Prandtl numbers
- 2013
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Ingår i: International Journal of Heat and Mass Transfer. - : Elsevier BV. - 0017-9310. ; 66, s. 164-176
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Tidskriftsartikel (refereegranskat)abstract
- Large Eddy Simulation and Direct Numerical Simulation are applied to study the turbulent flow field in a wavy channel at two Prandtl numbers, Pr = 0.71 and Pr = 3.5, and Reynolds number Re b = 10,000. The characteristics of the separated shear layer and the near wall recirculating zone are discussed in relation to the turbulent heat transfer. Special attention is paid to the behavior of the flow and thermal boundary layers and various turbulent characteristics and their effects on the distribution of the Nusselt number and friction coefficient in the separation and reattachment regions. The results indicate that the thickness of the thermal boundary layer rather than the turbulent fluctuations has a significant effect on the local variation of the averaged Nusselt number. The results are compared with Direct Numerical Simulation results of a plane channel at the same Reynolds number. © 2013 Elsevier Ltd. All rights reserved.
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| 4. |
- Purwandari, F. A., et al.
(författare)
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Pretreatment of oil palm empty fruit bunch (OPEFB) by N-methylmorpholine-N-oxide (NMMO) for biogas production: Structural changes and digestion improvement
- 2013
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Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 128, s. 461-466
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Tidskriftsartikel (refereegranskat)abstract
- Pretreatment of OPEFB (oil palm empty fruit bunch) by NMMO (N-methylmorpholine-N-oxide) on its subsequent digestions was investigated. The pretreatments were carried out at 90 and 120 degrees C for 1, 3, and 5 h in three different modes of dissolution (by 85% NMMO solution), ballooning (79% NMMO solution), and swelling (73% NMMO solution). The total solid recovery after the pretreatment was 89-94%. The pretreatment process did not have a major impact on the composition of OPEFB, other than a reduction of ash from 5.4% up to 1.3%. The best improvement in biogas production was achieved by a dissolution mode pretreatment of OPEFB, using conditions of 85% NMMO, 3 h, and 120 degrees C. It resulted in 0.408 Nm(3)/kg VS methane yield and 0.032 Nm(3) CH4/kg VS/day initial methane production rate, which correspond in improving by 48% and 167% compared to the untreated OPEFB, respectively.
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