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Interleukin-13 Trea...
Interleukin-13 Treatment of Living Lung Tissue Model Alters the Metabolome and Proteome : A Nano-DESI MS Metabolomics and Shotgun Proteomics Study
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- Tóth, Gábor (author)
- Uppsala universitet,Analytisk kemi
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- Golubova, Anastasia, 1996- (author)
- Uppsala universitet,Analytisk kemi
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- Falk, Alexander, Research engineer, 1986- (author)
- Uppsala universitet,Analytisk kemi
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- Bergström Lind, Sara, 1977- (author)
- Uppsala universitet,Analytisk kemi
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- Nicholas, Mark (author)
- AstraZeneca R&D, S-43183 Mölndal, Sweden.
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- Lanekoff, Ingela, Prof. 1975- (author)
- Uppsala universitet,Analytisk kemi
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(creator_code:org_t)
- MDPI, 2024
- 2024
- English.
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In: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 25:9
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Subject headings
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- Asthma is a chronic respiratory disease with one of the largest numbers of cases in the world; thus, constant investigation and technical development are needed to unravel the underlying biochemical mechanisms. In this study, we aimed to develop a nano-DESI MS method for the in vivo characterization of the cellular metabolome. Using air-liquid interface (ALI) cell layers, we studied the role of Interleukin-13 (IL-13) on differentiated lung epithelial cells acting as a lung tissue model. We demonstrate the feasibility of nano-DESI MS for the in vivo monitoring of basal-apical molecular transport, and the subsequent endogenous metabolic response, for the first time. Conserving the integrity of the ALI lung-cell layer enabled us to perform temporally resolved metabolomic characterization followed by "bottom-up" proteomics on the same population of cells. Metabolic remodeling was observed upon histamine and corticosteroid treatment of the IL-13-exposed lung cell monolayers, in correlation with alterations in the proteomic profile. This proof of principle study demonstrates the utility of in vivo nano-DESI MS for characterizing ALI tissue layers, and the new markers identified in our study provide a good starting point for future, larger-scale studies.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- asthma
- interleukin-13
- nano-DESI
- proteomics
- metabolomics
- multi-omics
- air-liquid interface
- amino acid
Publication and Content Type
- ref (subject category)
- art (subject category)
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