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Träfflista för sökning "WFRF:(Falk Johan) srt2:(1975-1999)"

Sökning: WFRF:(Falk Johan) > (1975-1999)

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  • Ivarsson, Marie-Louise, 1956, et al. (författare)
  • Characterization and fibrinolytic properties of mesothelial cells isolated from peritoneal lavage.
  • 1998
  • Ingår i: Scandinavian journal of clinical and laboratory investigation. - 0036-5513. ; 58:3, s. 195-203
  • Tidskriftsartikel (refereegranskat)abstract
    • Human peritoneal mesothelial cells were harvested from patients undergoing open or laparoscopic surgery for non-septic conditions using three different approaches: (1) from a peritoneal biopsy, (2) from peritoneal fluid, and (3) from lavage fluid collected from peritoneal cavity. When these different methods were compared, cells derived from peritoneal fluid or lavage were more likely to result in established cultures than those obtained from biopsies. The cells displayed morphological, immunohistochemical and ultrastructural characteristics of mesothelial cells. The cultured mesothelial cells produced tissue type plasminogen activator (t-PA), urokinase plasminogen activator (uPA), and plasminogen activator inhibitor type-1 and type-2 (PAI-1 and PAI-2) during unstimulated conditions. Treatment with the proinflammatory mediators LPS and TNF-alpha resulted in an overall decreased fibrinolytic capacity with a decrease in the release of t-PA and an increase in plasminogen activator inhibitors PAI-1 and PAI-2. TNF-alpha had a more profound effect than LPS, especially on the release of t-PA. This may be an important mechanism by which inflammatory mediators disrupt the fibrin degradation. In conclusion, peritoneal lavage is a convenient and reproducible source of mesothelial cells for culture.
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