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- Fall, Tove, 1979-, et al.
(författare)
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Diabetes Mellitus in Elkhounds Is Associated with Diestrus and Pregnancy
- 2010
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Ingår i: Journal of Veterinary Internal Medicine. - : Wiley. - 0891-6640 .- 1939-1676. ; 24:6, s. 1322-1328
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Tidskriftsartikel (refereegranskat)abstract
- Background: Female Elkhounds are shown to be at increased risk for diabetes mellitus, and occurrence of diabetes during pregnancy has been described in several cases. Hypothesis: Onset of diabetes mellitus in Elkhounds is associated with diestrus. Animals: Sixty-three Elkhounds with diabetes mellitus and 26 healthy controls. Methods: Medical records from 63 Elkhounds with diabetes were reviewed and owners were contacted for follow-up information. Blood samples from the day of diagnosis were available for 26 dogs. Glucose, fructosamine, C-peptide, growth hormone (GH), insulin-like growth factor-1, progesterone, and glutamate decarboxylase isoform 65-autoantibodies were analyzed and compared with 26 healthy dogs. Logistic models were used to evaluate the association of clinical variables with the probability of diabetes and with permanent diabetes mellitus after ovariohysterectomy (OHE). Results: All dogs in the study were intact females and 7 dogs (11%) were pregnant at diagnosis. The 1st clinical signs of diabetes mellitus occurred at a median of 30 days (interquartile range [IQR], 3-45) after estrus, and diagnosis was made at a median of 46 days (IQR, 27-62) after estrus. Diabetes was associated with higher concentrations of GH and lower concentrations of progesterone compared with controls matched for time after estrus. Forty-six percent of dogs that underwent OHE recovered from diabetes with a lower probability of remission in dogs with higher glucose concentrations (odds ratio [OR], 1.2; P = .03) at diagnosis and longer time (weeks) from diagnosis to surgery (OR, 1.5; P = .05). Conclusions: Diabetes mellitus in Elkhounds develops mainly during diestrus and pregnancy. Immediate OHE improves the prognosis for remission of diabetes.
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| 2. |
- Ahlgren, Kerstin M, et al.
(författare)
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Lack of evidence for a role of islet autoimmunity in the aetiology of canine diabetes mellitus
- 2014
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 9:8, s. e105473-
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Tidskriftsartikel (refereegranskat)abstract
- AIMS/HYPOTHESIS:Diabetes mellitus is one of the most common endocrine disorders in dogs and is commonly proposed to be of autoimmune origin. Although the clinical presentation of human type 1 diabetes (T1D) and canine diabetes are similar, the aetiologies may differ. The aim of this study was to investigate if autoimmune aetiology resembling human T1D is as prevalent in dogs as previously reported.METHODS:Sera from 121 diabetic dogs representing 40 different breeds were tested for islet cell antibodies (ICA) and GAD65 autoantibodies (GADA) and compared with sera from 133 healthy dogs. ICA was detected by indirect immunofluorescence using both canine and human frozen sections. GADA was detected by in vitro transcription and translation (ITT) of human and canine GAD65, followed by immune precipitation. Sections of pancreata from five diabetic dogs and two control dogs were examined histopathologically including immunostaining for insulin, glucagon, somatostatin and pancreas polypeptide.RESULTS:None of the canine sera analysed tested positive for ICA on sections of frozen canine or human ICA pancreas. However, serum from one diabetic dog was weakly positive in the canine GADA assay and serum from one healthy dog was weakly positive in the human GADA assay. Histopathology showed marked degenerative changes in endocrine islets, including vacuolisation and variable loss of immune-staining for insulin. No sign of inflammation was noted.CONCLUSIONS/INTERPRETATIONS:Contrary to previous observations, based on results from tests for humoral autoreactivity towards islet proteins using four different assays, and histopathological examinations, we do not find any support for an islet autoimmune aetiology in canine diabetes mellitus.
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| 4. |
- Blanco Penedo, Isabel, et al.
(författare)
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Comparison of Bovine coronavirus-specific and Bovine respiratory syncytial virus-specific antibodies in serum versus milk samples detected by enzyme-linked immunosorbent assay
- 2014
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Ingår i: Journal of Veterinary Diagnostic Investigation. - : SAGE Publications. - 1040-6387 .- 1943-4936. ; 26, s. 113-116
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Tidskriftsartikel (refereegranskat)abstract
- Bovine coronavirus (BCV; Betacoronavirus 1) and Bovine respiratory syncytial virus (BRSV) are significant causes of enteric and respiratory disease in beef and dairy cattle throughout the world. Indirect enzyme-linked immunosorbent assays are widely used to detect serum antibodies for herd monitoring and prevalence studies. In dairy herds, milk is more readily collected than serum. Hence, in order to investigate the test agreement between serum and milk, both serum and milk samples from 105 cows in 27 dairy herds were analyzed in parallel for presence of immunoglobulin G antibodies to BCV and BRSV. The Bland-Altman analyses of data demonstrated good agreement between serum and milk antibody titers for both viruses. The results indicate milk samples are sufficient for surveillance of antibodies to BCV and BRSV.
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| 5. |
- Blanco Penedo, Isabel, et al.
(författare)
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Effects of turning to 100% organic feed on metabolic status of Swedish organic dairy cows
- 2012
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Ingår i: Livestock Science. - : Elsevier BV. - 1871-1413 .- 1878-0490. ; 143, s. 242-248
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Tidskriftsartikel (refereegranskat)abstract
- Since January 2008 the ration for organic herds has been required to be 100% organic (Regulation No. 889/2008). However, this may not satisfy the high energy demands of early lactation cows. We thus investigated the possible effects of 100% organic feed on the energy balance in Swedish organic dairy herds as indicated by the following blood parameters (n = 739 observations): Beta-hydroxybutyrate (BHBA), non-esterified fatty acid (NEFA) and insulin, and the occurrence of clinical ketosis (n = 8769 observations). Thirteen organic and 13 conventional herds were visited and blood samples from 81 cows around parturition ( >= 3 cows per herd) were used. The metabolic status of the same herds under the previous rules was available for comparison. The BHBA, NEFA and insulin levels were different before and after the change of legislation, but the effects were similar in organic and conventional cows. The incidence of clinical ketosis was not associated with herd type or the change of legislation. Thus the change in legislation did not appear to have had any detrimental effects on the metabolic profiles of organic cows in early lactation and there was no evidence that organic cows were metabolically more challenged or had a severe negative energy balance. (C) 2011 Elsevier B.V. All rights reserved.
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| 6. |
- Blanco Penedo, Isabel, et al.
(författare)
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Exposure to pasture borne nematodes affects individual milk yield in Swedish dairy herds
- 2012
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Ingår i: Veterinary Parasitology. - : Elsevier BV. - 0304-4017 .- 1873-2550. ; 188, s. 93-98
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Tidskriftsartikel (refereegranskat)abstract
- The association between exposure to gastrointestinal nematode (GIN) parasitism, indicated by elevated individual optical density ratio (ODR) to Ostertagia ostertagi, and individual milk production was analysed in dairy cows. The multivariable analysis also accounted for other sources of variation than exposure to GIN parasitism. Intra- and inter-herd variation in parasite exposure was also evaluated. Organic and conventional herds located in south-east Sweden (13 herds per system) were visited during the housing period 2009-2010. Levels of all major pasture-borne helminths in Sweden (O. ostertagi, Fasciola hepatica and Dictyocaulus viviparus) were determined in bulk tank milk, cow milk and serum (only for O. ostertagi). Their specific antibodies were detected using three different ELISAs (Svanova Biotech) and the optical density of the sample was expressed as ODR. Positive cases of Dictyocaulus and Fasciola were seldom found. Variation in ODR in serum samples for Ostertagia was higher within herds than between herds. Correlations between ODR for Ostertagia in serum and milk parameters were significant and negative. In the multivariable analysis, two models were performed separating data from primiparous and multiparous cows. Results from mixed models showed that daily milk yield was significantly influenced by Ostertagia ODR for multiparous cows. No interactions were found between Ostertagia ODR values and herd type. (C) 2012 Elsevier B.V. All rights reserved.
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