| 1. |
- Robinson, N. A., et al.
(författare)
-
Applying genetic technologies to combat infectious diseases in aquaculture
- 2022
-
Ingår i: Reviews in Aquaculture. - : Wiley. - 1753-5123 .- 1753-5131. ; 15:2, s. 491-535
-
Tidskriftsartikel (refereegranskat)abstract
- Disease and parasitism cause major welfare, environmental and economic concerns for global aquaculture. In this review, we examine the status and potential of technologies that exploit genetic variation in host resistance to tackle this problem. We argue that there is an urgent need to improve understanding of the genetic mechanisms involved, leading to the development of tools that can be applied to boost host resistance and reduce the disease burden. We draw on two pressing global disease problems as case studies—sea lice infestations in salmonids and white spot syndrome in shrimp. We review how the latest genetic technologies can be capitalised upon to determine the mechanisms underlying inter- and intra-species variation in pathogen/parasite resistance, and how the derived knowledge could be applied to boost disease resistance using selective breeding, gene editing and/or with targeted feed treatments and vaccines. Gene editing brings novel opportunities, but also implementation and dissemination challenges, and necessitates new protocols to integrate the technology into aquaculture breeding programmes. There is also an ongoing need to minimise risks of disease agents evolving to overcome genetic improvements to host resistance, and insights from epidemiological and evolutionary models of pathogen infestation in wild and cultured host populations are explored. Ethical issues around the different approaches for achieving genetic resistance are discussed. Application of genetic technologies and approaches has potential to improve fundamental knowledge of mechanisms affecting genetic resistance and provide effective pathways for implementation that could lead to more resistant aquaculture stocks, transforming global aquaculture. © 2022 The Authors. Reviews in Aquaculture published by John Wiley & Sons Australia, Ltd.
|
|
| 2. |
- Fast, L, et al.
(författare)
-
Fluorescence-Based Measurements of Membrane-Bound Angiotensin Converting Enzyme 2 Activity Using Xenopus Laevis Oocytes
- 2022
-
Ingår i: Biosensors. - : MDPI AG. - 2079-6374. ; 12:8
-
Tidskriftsartikel (refereegranskat)abstract
- Functional investigations of enzymes involving cellular expression systems are important for pharmacological studies. The precise control of expression is challenging in transiently transfected mammalian cell lines. Here, we explored the ability of Xenopus laevis oocytes to express a membrane-bound enzyme for functional characterization using standard 96-well plates and a fluorescence-based plate reader assay. We microinjected oocytes with cRNA encoding the angiotensin converting enzyme 2 (ACE2) and measured the enzymatic activity in single oocytes using a commercial fluorescence-based assay. The injected oocytes showed up to a 50-fold increase in fluorescence compared to uninjected oocytes. This fluorescence intensity was dose-dependent on the amount of ACE2 cRNA. These results suggest that Xenopus oocytes can be used for the functional evaluation of membrane-bound enzymes, decreasing the experimental workload.
|
|
| 3. |
- Pettersson, Pär, 1985, et al.
(författare)
-
Intrinsic differences between backward and forward vehicle simulation models
- 2020
-
Ingår i: IFAC-PapersOnLine. - : Elsevier. - 2405-8963. ; 53
-
Konferensbidrag (refereegranskat)abstract
- Two common methods for predicting the energy usage in vehicles through mathematical simulation, the 'backward' and the 'forward' schemes, are discussed and compared in terms of the longitudinal vehicle behaviour they predict. In the backward scheme, the input driving cycle is initially assumed to be followed perfectly and therefore the vehicle speed is not a dynamic state. In the forward scheme, a driver model controls the vehicle in an attempt to follow the input driving cycle, and the vehicle speed is intrinsically a dynamic state. A theoretical study is made with a simple mathematical vehicle model, where it is shown that the two methods neither predict the same expected energy use nor energy variation. Next, the simulation model that is used for the CO2 rating of heavy-duty trucks in Europe, VECTO, is used as an example of the backward method, and an equivalent implementation in a forward scheme is attempted. Two numerical experiments are made with these models: a detailed study of the longitudinal vehicle behaviour on a reference mission; and a study of the predicted CO2 emissions on a family of stochastically generated missions. The conclusion is that the backward method is easier to use but the forward method has a greater potential to predict realistic behaviour.
|
|
| 4. |
- Sampath, Vanitha, et al.
(författare)
-
Vaccines and allergic reactions : The past, the current COVID-19 pandemic, and future perspectives
- 2021
-
Ingår i: Allergy: European Journal of Allergy and Clinical Immunology. - : Wiley. - 0105-4538. ; 76:6, s. 1640-1660
-
Forskningsöversikt (refereegranskat)abstract
- Vaccines are essential public health tools with a favorable safety profile and prophylactic effectiveness that have historically played significant roles in reducing infectious disease burden in populations, when the majority of individuals are vaccinated. The COVID-19 vaccines are expected to have similar positive impacts on health across the globe. While serious allergic reactions to vaccines are rare, their underlying mechanisms and implications for clinical management should be considered to provide individuals with the safest care possible. In this review, we provide an overview of different types of allergic adverse reactions that can potentially occur after vaccination and individual vaccine components capable of causing the allergic adverse reactions. We present the incidence of allergic adverse reactions during clinical studies and through post-authorization and post-marketing surveillance and provide plausible causes of these reactions based on potential allergenic components present in several common vaccines. Additionally, we review implications for individual diagnosis and management and vaccine manufacturing overall. Finally, we suggest areas for future research.
|
|
