| 1. |
- Cossarizza, A., et al.
(författare)
-
Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
- 2019
-
Ingår i: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 49:10, s. 1457-1973
-
Tidskriftsartikel (refereegranskat)abstract
- These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
|
|
| 2. |
|
|
| 3. |
- Olalde, I., et al.
(författare)
-
The Beaker phenomenon and the genomic transformation of northwest Europe
- 2018
-
Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 555:7695, s. 190-196
-
Tidskriftsartikel (refereegranskat)abstract
- From around 2750 to 2500 bc, Bell Beaker pottery became widespread across western and central Europe, before it disappeared between 2200 and 1800 bc. The forces that propelled its expansion are a matter of long-standing debate, and there is support for both cultural diffusion and migration having a role in this process. Here we present genome-wide data from 400 Neolithic, Copper Age and Bronze Age Europeans, including 226 individuals associated with Beaker-complex artefacts. We detected limited genetic affinity between Beaker-complex-associated individuals from Iberia and central Europe, and thus exclude migration as an important mechanism of spread between these two regions. However, migration had a key role in the further dissemination of the Beaker complex. We document this phenomenon most clearly in Britain, where the spread of the Beaker complex introduced high levels of steppe-related ancestry and was associated with the replacement of approximately 90% of Britain's gene pool within a few hundred years, continuing the east-to-west expansion that had brought steppe-related ancestry into central and northern Europe over the previous centuries.
|
|
| 4. |
|
|
| 5. |
- Ferry, Sven A., et al.
(författare)
-
High diagnostic accuracy of nitrite test paired with Urine sediment can reduce unnecessary antibiotic therapy
- 2015
-
Ingår i: Open Microbiology Journal. - : Bentham Science Publishers Ltd.. - 1874-2858. ; 9, s. 150-159
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Urinary tract infections (UTIs) are common bacterial infections dominated by lower UTI in women (LUTIW). Symptoms only are insufficient for diagnosis and accordingly, near patient diagnostic tests confidently confirming significant bacteriuria are desirable. The nitrite test (NIT) has low sensitivity, while bacterial and leukocyte counts disjunctively paired in urine sediment microscopy (SED) have high sensitivity. Similar symptomatic cure rates are found post antibiotic vs. placebo therapy in patients with negative cultures. Consequently, prescription on symptoms only implies unnecessary antibiotic therapy. Aims: to evaluate the diagnostic outcomes of NIT, SED and NIT disjunctively paired with SED (NIT+SED) vs. urine culture, with special focus on bladder incubation time (BIT), and to assess if NIT+SED can reduce unnecessary antibiotic therapy. Methods: A diagnostic, primary care, multicentre study including 1070 women with symptoms suggestive of lower UTI. Results: Significant bacteriuria was found in 77%. The BIT highly influenced the diagnostic outcomes and the optimal duration was ≥4h with sensitivity of 66, 90 and 95% for NIT, SED and NIT+SED, respectively. SED performed only in NIT negative specimens could reduce unnecessary antibiotics by 10% vs. prescription on symptoms only. The number needed to test with SED to reduce one unnecessary antibiotic course was five patients at BIT ≥4h and six patients at ≤3h or overall. Conclusion: The BIT highly influences the diagnostic outcomes with the highest accuracy of NIT+SED. Diagnosis of LUTIW with NIT+SED can reduce unnecessary antibiotic therapy and subsequently decrease antimicrobial resistance. Trial registration: The Swedish Medical Product Agency 1995 03 01:151:01783/94. © 2015, Ferry et al.; Licensee Bentham Open.
|
|
| 6. |
|
|
| 7. |
- Kadkhodazadeh, S., et al.
(författare)
-
Optical Property-Composition Correlation in Noble Metal Alloy Nanoparticles Studied with EELS
- 2019
-
Ingår i: ACS Photonics. - : American Chemical Society (ACS). - 2330-4022. ; 6:3, s. 779-786
-
Tidskriftsartikel (refereegranskat)abstract
- Noble metals are currently the most common building blocks in plasmonics and thus define the available range of optical properties. Their alloying provides a viable strategy to engineer new materials with a tunable range of optical responses. Despite this attractive prospect, the link between composition and optical properties of many noble metal alloys is still not well understood. Here, electron energy-loss spectroscopy is employed to systematically study AuAg and AuPd nanoparticles of varying compositions. The localized surface plasmons, the bulk plasmons, and the permittivity functions of these two sets of alloys are investigated as functions of their composition. In the case of the more widely studied AuAg alloy system, good agreement is found with previous experimental and theoretical studies. The results on the less scrutinized AuPd system provide highly valuable experimental data that complements other experimental investigations and supports the development of theoretical models.
|
|
| 8. |
|
|
