| 1. |
- Gustafson, Stefan, et al.
(författare)
-
Accessible hyaluronan receptors identical to ICAM-1 in mouse mast-cell tumours
- 1995
-
Ingår i: Glycoconjugate Journal. - 0282-0080 .- 1573-4986. ; 12:3, s. 350-355
-
Tidskriftsartikel (refereegranskat)abstract
- Immunohistochemical studies of the hyaluronan (HA)-receptor (R), originally found on liver endothelial cells (LEC) and related to the intercellular adhesion molecule 1 (ICAM-1), showed that polyclonal antibodies against HARLEC (HA receptor on LEC) also stain structures in mouse mastocytomas, mainly vessels. To test if intravenously administered HA might target the tumour receptors in vivo, mice carrying an inoculated mastocytoma in one hind leg muscle were injected in the tail vein with 125I-tyrosine (T)-labelled HA and killed 75 min after injection when organs and tissues were checked for radioactivity. When doses exceeding the binding capacity of the liver were injected, a significant increase in radioactivity (up to five-fold) within the tumour tissue was found. The weight adjusted difference between control and tumour tissue was greater for smaller tumours, probably due to necrosis in the larger. HA-staining of tumours from animals receiving 125I-T-HA, showed HA in areas that also stained weakly for ICAM-1 using monoclonal antibodies. ICAM-1 staining was dramatically increased after hyaluronidase treatment of the sections, indicating that the HA is bound to these receptors and thereby blocks antibody recognition.
|
|
| 2. |
- Persson, Cathrine, et al.
(författare)
-
Localization of the Yersinia PTPase to focal complexes is an important virulence mechanism
- 1999
-
Ingår i: Molecular Microbiology. - : Wiley-Blackwell Publishing Inc.. - 0950-382X .- 1365-2958. ; 33:4, s. 828-838
-
Tidskriftsartikel (refereegranskat)abstract
- The protein tyrosine phosphatase YopH, produced by the pathogen Yersinia pseudotuberculosis, is an essential virulence determinant involved in antiphagocytosis. Upon infection, YopH is translocated into the target cell, where it recognizes focal complexes. Genetic analysis revealed that YopH harbours a region that is responsible for specific localization of this PTPase to focal complexes in HeLa cells and professional phagocytes. This region is a prerequisite for blocking an immediate-early Yersinia-induced signal within target cells. The region is also essential for antiphagocytosis and virulence, illustrating the biological significance of localization of YopH to focal complexes during Yersinia infection. These results also indicate that focal complexes play a role in the general phagocytic process.
|
|
