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Results of a collab...
Results of a collaborative study on DNA identification of aged bone samples
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- Vanek, Daniel (författare)
- Forens DNA Serv Prague, Prague, Czech Republic.;Charles Univ Prague, Fac Med 2, Prague, Czech Republic.
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- Budowle, Bruce (författare)
- Univ North Texas, Hlth Sci Ctr, Ctr Human Identificat, Ft Worth, TX 76107 USA.;King Abdulaziz Univ, Ctr Excellence Genom Med Res CEGMR, Jeddah, Saudi Arabia.
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- Dubska-Votrubova, Jitka (författare)
- Forens DNA Serv Prague, Prague, Czech Republic.
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- Ambers, Angie (författare)
- Univ North Texas, Hlth Sci Ctr, Ctr Human Identificat, Ft Worth, TX 76107 USA.
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- Frolik, Jan (författare)
- Inst Archaeol CR, Prague, Czech Republic.
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- Pospisek, Martin (författare)
- Biologicals, Ricany, Czech Republic.;Charles Univ Prague, Fac Sci, Prague, Czech Republic.
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- Al Afeefi, Ahmed Anwar (författare)
- Minist Interior, Abu Dhabi Police Gen Directorate, Forens Evidence Dept, Abu Dhabi, U Arab Emirates.
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- Al Hosani, Khalid Ismaeil (författare)
- Minist Interior, Abu Dhabi Police Gen Directorate, Forens Evidence Dept, Abu Dhabi, U Arab Emirates.
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- Allen, Marie (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi
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- Al Naimi, Khudooma Saeed (författare)
- Minist Interior, Abu Dhabi Police Gen Directorate, Forens Evidence Dept, Abu Dhabi, U Arab Emirates.
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- Al Salafi, Dina (författare)
- Minist Interior, Sharjah Police HQ, Sharjah Forens Lab, Abu Dhabi, U Arab Emirates.
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- Al Tayyari, Wafa Ali Rashid (författare)
- Minist Interior, Abu Dhabi Police Gen Directorate, Forens Evidence Dept, Abu Dhabi, U Arab Emirates.
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- Arguetaa, Wendy (författare)
- FAFG, Forens Genet Dept, Guatemala City, Guatemala.
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- Bottinelli, Michel (författare)
- Lab Diagnost Mol, Gentilino, Switzerland.
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- Bus, Magdalena M. (författare)
- Uppsala universitet,Medicinsk genetik och genomik,Science for Life Laboratory, SciLifeLab
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- Cemper-Kiesslich, Jan (författare)
- Salzburg Univ, Interfaculty Dept Legal Med, Salzburg, Austria.
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- Cepil, Olivier (författare)
- Biomnis, Lyon, France.
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- De Cock, Greet (författare)
- Natl Inst Criminalistiek Criminol, Brussels, Belgium.
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- Desmyter, Stijn (författare)
- Natl Inst Criminalistiek Criminol, Brussels, Belgium.
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- El Amri, Hamid (författare)
- Genet Lab Royal Gendarmerie, Rabat, Morocco.
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- El Ossmani, Hicham (författare)
- Genet Lab Royal Gendarmerie, Rabat, Morocco.
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- Galdies, Ruth (författare)
- Univ Malta, Lab Mol Genet, Biomed Sci Bldg, Msida, Malta.;Univ Malta, Malta BioBank, Dept Phsyiol & Biochem, Biomed Sci Bldg, Msida, Malta.
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- Gruen, Sebastian (författare)
- Bayer Landeskriminalamt, Munich, Germany.
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- Guidet, Francois (författare)
- Azur Genet, Nice, France.
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- Hoefges, Anna (författare)
- Univ Malta, Lab Mol Genet, Biomed Sci Bldg, Msida, Malta.;Univ Malta, Malta BioBank, Dept Phsyiol & Biochem, Biomed Sci Bldg, Msida, Malta.
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- Iancu, Cristian Bogdan (författare)
- Nat Inst Legal Med Mina Minovici, Bucharest, Romania.
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Lotz, Petra (författare)
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- Maresca, Alessandro (författare)
- Lab Diagnost Mol, Gentilino, Switzerland.
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- Nagy, Marion (författare)
- Charite, Inst Legal Med & Forens Sci, Dept Forens Genet, Berlin, Germany.
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- Novotny, Jindrich (författare)
- Bayer Landeskriminalamt, Munich, Germany.
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- Rachid, Hajar (författare)
- Genet Lab Royal Gendarmerie, Rabat, Morocco.
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- Rothe, Jessica (författare)
- Charite, Inst Legal Med & Forens Sci, Dept Forens Genet, Berlin, Germany.
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- Stenersen, Marguerethe (författare)
- Norwegian Inst Publ Hlth, Div Forens Sci Forens Genet, Oslo, Norway.
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- Stephenson, Mishel (författare)
- FAFG, Forens Genet Dept, Guatemala City, Guatemala.
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- Stevanovitch, Alain (författare)
- Inst Natl Police Sci, Lab Police Sci Marseille, Marseille, France.
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- Strien, Juliane (författare)
- Friedrich Schiller Univ Jena, Jena Univ Hospital, Inst Legal Med, Jena, Germany.
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- Sumita, Denilce R. (författare)
- Genom Engn Mol, Sao Paulo, Brazil.
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- Vella, Joanna (författare)
- Univ Malta, Lab Mol Genet, Biomed Sci Bldg, Msida, Malta.;Univ Malta, Malta BioBank, Dept Phsyiol & Biochem, Biomed Sci Bldg, Msida, Malta.
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Zander, Judith (författare)
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Forens DNA Serv Prague, Prague, Czech Republic;Charles Univ Prague, Fac Med 2, Prague, Czech Republic. Univ North Texas, Hlth Sci Ctr, Ctr Human Identificat, Ft Worth, TX 76107 USA.;King Abdulaziz Univ, Ctr Excellence Genom Med Res CEGMR, Jeddah, Saudi Arabia. (creator_code:org_t)
- MEDICINSKA NAKLADA, 2017
- 2017
- Engelska.
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Ingår i: Croatian Medical Journal. - : MEDICINSKA NAKLADA. - 0353-9504 .- 1332-8166. ; 58:3, s. 203-213
- Relaterad länk:
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https://doi.org/10.3...
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visa fler...
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https://www.ncbi.nlm...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Aim: A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples.Methods: Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory.Results: Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality.Conclusion: The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.
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Vanek, Daniel
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Budowle, Bruce
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Dubska-Votrubova ...
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Ambers, Angie
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Frolik, Jan
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Pospisek, Martin
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visa fler...
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Al Afeefi, Ahmed ...
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Al Hosani, Khali ...
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Allen, Marie
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Al Naimi, Khudoo ...
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Al Salafi, Dina
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Al Tayyari, Wafa ...
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Arguetaa, Wendy
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Bottinelli, Mich ...
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Bus, Magdalena M ...
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Cemper-Kiesslich ...
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Cepil, Olivier
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De Cock, Greet
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Desmyter, Stijn
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El Amri, Hamid
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El Ossmani, Hich ...
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Galdies, Ruth
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Gruen, Sebastian
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Guidet, Francois
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Hoefges, Anna
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Iancu, Cristian ...
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Lotz, Petra
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Maresca, Alessan ...
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Nagy, Marion
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Novotny, Jindric ...
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Rachid, Hajar
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Rothe, Jessica
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Stenersen, Margu ...
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Stephenson, Mish ...
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Stevanovitch, Al ...
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Strien, Juliane
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Sumita, Denilce ...
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Vella, Joanna
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Zander, Judith
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Croatian Medical ...
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Uppsala universitet