| 1. |
- Garcia-Alonso, Luz, et al.
(författare)
-
Mapping the temporal and spatial dynamics of the human endometrium in vivo and in vitro
- 2021
-
Ingår i: Nature Genetics. - : Springer Nature. - 1061-4036 .- 1546-1718. ; 53:12, s. 1698-
-
Tidskriftsartikel (refereegranskat)abstract
- The endometrium, the mucosal lining of the uterus, undergoes dynamic changes throughout the menstrual cycle in response to ovarian hormones. We have generated dense single-cell and spatial reference maps of the human uterus and three-dimensional endometrial organoid cultures. We dissect the signaling pathways that determine cell fate of the epithelial lineages in the lumenal and glandular microenvironments. Our benchmark of the endometrial organoids reveals the pathways and cell states regulating differentiation of the secretory and ciliated lineages both in vivo and in vitro. In vitro downregulation of WNT or NOTCH pathways increases the differentiation efficiency along the secretory and ciliated lineages, respectively. We utilize our cellular maps to deconvolute bulk data from endometrial cancers and endometriotic lesions, illuminating the cell types dominating in each of these disorders. These mechanistic insights provide a platform for future development of treatments for common conditions including endometriosis and endometrial carcinoma. Single-cell and spatial transcriptomic profiling of the human endometrium highlights pathways governing the proliferative and secretory phases of the menstrual cycle. Analyses of endometrial organoids show that WNT and NOTCH signaling modulate differentiation into the secretory and ciliated epithelial lineages, respectively.
|
|
| 2. |
- Theland, Freddie, et al.
(författare)
-
Assessment of small-strain characteristics for vibration predictions in a Swedish clay deposit
- 2021
-
Ingår i: Soil Dynamics and Earthquake Engineering. - : Elsevier BV. - 0267-7261 .- 1879-341X. ; 150
-
Tidskriftsartikel (refereegranskat)abstract
- Environmental vibrations induced by human activities such as traffic, construction or industrial manufacturing can cause disturbance among residents or to vibration sensitive equipment in buildings. In Sweden, geological formations of soft clay overlying a stiff bedrock are soil conditions prone to ground vibrations that are encountered both in urban areas and along parts of the national railway network. This paper presents an extensive investigation of the small-strain soil properties for the prediction of environmental ground vibrations in a shallow clay where the bedrock is situated at 7.5 m depth. The small-strain properties are estimated using available empirical correlations, bender elements, seismic cone penetration tests, seismic refraction and inversion of surface wave dispersion and attenuation curves. The results are synthesised into a dynamic layered soil model which is validated by measurements at the soil's surface at source-receiver distances up to 90 m in the frequency range 1-80 Hz. Analyses of uncertainties in the estimated values of wave speeds and material damping are performed by model investigations, indicating that surface wave tests overestimate the damping compared to bender element tests. The properties of the topmost unsaturated part of the soil is found to have a significant influence on the response at large distances, caused by critically refracted P-waves resonating in the top layer.
|
|
| 3. |
- Zangeneh, Abbas, 1980-, et al.
(författare)
-
Modal analysis of coupled soil-structure systems
- 2021
-
Ingår i: Soil Dynamics and Earthquake Engineering. - : Elsevier BV. - 0267-7261 .- 1879-341X. ; 144
-
Tidskriftsartikel (refereegranskat)abstract
- This paper presents an efficient approach for the modal analysis of coupled soil-structure systems, for which the dynamic response is strongly influenced by the embedment in the soil. The methodology is based on a finite element-perfectly matched layer model that allows for the derivation of frequency-independent system matrices and the computation of the modal properties of the coupled system. This is achieved by solving a nonlinear eigenproblem using a Compact Rational Krylov (CoRK) eigensolver. A procedure is developed to sort the computed eigenpairs, filter out the spurious modes of the system which are related to the near-field and truncated far-field soil subdomains and select the physical structural modes of system. The proposed method can be used in the dynamic assessment and structural identification of strongly coupled soil-structure systems such as fully or partially buried structures and allows for the interpretation of experimentally identified modal properties of these systems, especially in the presence of highly damped or closely spaced coupled modes. The applicability and the scalability of the proposed approach for 2D and 3D problems is demonstrated in two case studies.
|
|
