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- Lundgren, T, et al.
(författare)
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Parathyroid hormone (1-34) receptor-binding and second-messenger response in rat incisor odontoblasts.
- 1998
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Ingår i: Calcified tissue international. - 0171-967X. ; 62:3, s. 255-9
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Tidskriftsartikel (refereegranskat)abstract
- Even though indirect evidence indicates that PTH exerts an anabolic effect on dentinogenesis, the existence of PTH receptors and any second-messenger response in odontoblasts have not been demonstrated. The aim of this study was to investigate whether rat incisor odontoblasts express PTH receptors, and to identify which second messenger pathway the hormone may activate. Odontoblasts were dissected from rat incisors. Amino-terminal (1-34) fragment rat PTH [rPTH(1-34)] conjugated to fluorescein isothiocyanate visualized receptor sites on the cell surface. Upon incubation of odontoblasts with rPTH(1-34), cAMP formation was increased. However, no fluctuations in intracellular calcium activity were observed upon rPTH(1-34) stimulation when using Fura-2 as a Ca2+ probe. In long-time incubations, stimulation with PTH(1-34) upregulated APase activity. The results demonstrate that rPTH(1-34) evokes an anabolic response in dentinogenically active odontoblasts, and that this may be mediated through the protein kinase A/cAMP pathway, whereas no indications for Ca2+ as a second messenger were evident.
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- Treshchev, S.Y., et al.
(författare)
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Determination of osmium by laser-induced fluorescence in a graphite furnace in combination with an intensified charge coupled device
- 1999
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Ingår i: Journal of Analytical Chemistry. - : Pleiades Publishing. - 1061-9348 .- 1608-3199. ; 54:3, s. 279-282
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Tidskriftsartikel (refereegranskat)abstract
- Laser-induced fluorescence in a graphite furnace with an intensified diode array is used for determining ultratrace osmium. Fluorescence spectra of osmium were investigated in a wide spectral range for five excitation transitions from the ground state. The optimum wavelengths of excitation and fluorescence were selected; the limit of detection of osmium in aqueous solutions is 50 pg.
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- Nuber, Ulrike, et al.
(författare)
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The widespread human desmocollin Dsc2 and tissue-specific patterns of synthesis of various desmocollin subtypes
- 1995
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Ingår i: European Journal of Cell Biology. - 0171-9335. ; 66:1, s. 69-74
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Tidskriftsartikel (refereegranskat)abstract
- By comparison of the cDNA-derived amino acid sequences and the cell type-specific patterns of synthesis we have identified desmocollin Dsc2 as the most widespread, perhaps ubiquitous desmocollin subtype. Using Northern blot analyses and ribonuclease protection assays we have found an approximately 5.6 kb mRNA encoding Dsc2 in all the diverse human tissues, tumors and cell lines examined that are known to possess desmosomes, i.e. not only epithelial cells but also myocardiac cells and lymph nodes. By contrast, desmocollin subtypes Dsc1 and Dsc3 have been detected only in certain stratified squamous epithelia, with the most conspicuous restriction of Dsc1 to epidermis and--remarkably, but unexplained--lymph nodes, and in certain carcinomas and cell lines derived therefrom. We have also determined that both Dsc2 mRNA splice forms, the one encoding the larger polypeptide a and the one coding for the shorter Dsc2b, occur in all the diverse tissues and cell lines examined. We also show that certain cells such as the epidermal keratinocyte line HaCaT and the vulvar carcinoma-derived line A-431 continually synthesize more than one Dsc subtype. The cell type-specific patterns of synthesis of the various Dsg and Dsc subtypes are discussed in relation to tissue development during embryogenesis and to malignant transformations, and the utilization of reagents for the specific Dsg and Dsc subtypes in tumor diagnosis is proposed.
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