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| 2. |
- Cheng, Fang, et al.
(författare)
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Patterns of uronosyl epimerization and 4-/6-O-sulphation in chondroitin/dermatan sulphate from decorin and biglycan of various bovine tissues
- 1994
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Ingår i: Glycobiology. - 1460-2423. ; 4:5, s. 685-696
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Tidskriftsartikel (refereegranskat)abstract
- Dermatan sulphate is a co-polymer of two types of disaccharide repeats: D-glucuronate-N-acetylgalactosamine and L-iduronate-N-acetylgalactosamine. The former can be O-sulphated at C-4 or C-6 of the galactosamine, whereas the latter contains almost exclusively 4-O-sulphated galactosamine. A minor proportion of the L-iduronate may be O-sulphated at C-2. Chondroitin sulphate has no L-iduronate-containing repeats. We have used our recently developed methods for sequence analysis of galactosaminoglycans to investigate the structure of dermatan/chondroitin sulphates of the proteoglycans decorin and biglycan derived from various bovine tissues, like dermis, sclera, tendon, aorta, cartilage and bone. The glycan chains, radioiodinated at the reducing end, were partially cleaved with specific enzymes (chondroitin lyases), and subjected to high-resolution polyacrylamide gel electrophoresis, blotting and autoradiography to identify fragments extending from the labelled reducing end to the point of cleavage. We used chondroitin B lyase to identify the location of L-iduronate, chondroitin AC-I lyase to locate D-glucuronate and chondroitin C lyase to cleave where D-glucuronate residues were succeeded by 6-O-sulphated N-acetylgalactosamine. We could demonstrate tissue-specific, periodic and wave-like patterns of distribution for the two epimeric uronic acids, as well as specific patterns of sulphation in dermatan sulphates derived from either decorin or biglycan. For example, some dermatan sulphates contained D-glucuronate-rich domains that were always 6-sulphated (scleral decorin), others were always 4-sulphated (decorin from bovine dermis, cartilage and bone; biglycan from aorta) or 6-sulphated near the linkage region, but 4-sulphated in more distal domains (decorin from porcine dermis and bovine tendon). Decorin from bone and articular cartilage, as well as biglycan from articular and nasal cartilage, carried largely chondroitin sulphate chains, but also some dermatan sulphate, whereas galactosaminoglycan chains derived from aggrecan of nasal cartilage were free of L-iduronate. Decorin and biglycan from the same tissue (articular cartilage or sclera) had similar glycan chains. The two side chains in a biglycan molecule are probably also similar to one another. The portion of the glycan chains nearest to the core protein was substituted with charged groups to a variable degree, which may correlate with the structural features of the main chain.
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| 4. |
- Fransson, Anders, et al.
(författare)
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Att ta steget till vuxenstudier
- 1990
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Ingår i: Studiesteg för vuxna. - Göteborg : Institutionen för pedagogik, Göteborgs universitet.
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Ett resonemang om hur man kan överbrygga studiehinder
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| 6. |
- Fransson, Lennart, et al.
(författare)
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Sea ice properties studied from the icebreaker Tor during BEPERS-88
- 1990
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The report presents sea ice data taken during BEPERS-88 experiment in the Bothnian Bay. Several physical properties are presented and analysed. In general, the data illustrate that sea ice is a most complex medium with horizontal and vertical variations. Any analysis of remotely sensed data must therefore carefully consider the ground truth data.
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| 7. |
- Fransson, Lars-Åke, et al.
(författare)
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Oligosaccharide mapping of proteoglycan-bound and xyloside-initiated dermatan sulfate from fibroblasts
- 1991
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Ingår i: Glycoconjugate Journal. - 1573-4986. ; 8:2, s. 108-115
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Tidskriftsartikel (refereegranskat)abstract
- The copolymeric structure of dermatan sulfate chains synthesized by skin fibroblasts has been examined. Chains initiated onto exogeneous p-nitrophenyl-beta-D-xylopyranoside or attached to protein in a large proteoglycan, PG-L, and two small proteoglycans, PG-S1 and PG-S2, have been compared by using high resolution electrophoresis and gel chromatography of oligosaccharides generated by specific enzymatic or chemical degradations. The results confirm that chains attached to PG-L are glucuronate-rich, whereas novel findings indicate that chains attached to either of the two PG-S variants yield closely similar oligosaccharide maps, have approximately equal glucuronate and iduronate content and contain over 90% 4-sulfated disaccharide repeating units. Dermatan sulfate chains built onto xyloside at concentrations of 50 microM and below have a copolymeric structure similar to that of chains from the two PG-S variants. These findings indicate that the polymer-modifying machinery can generate chains with extended iduronate-containing repeats also when the xylose primer is not linked to core protein.
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| 8. |
- Johansson, Rolf, et al.
(författare)
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Discrimination between Patients with Acoustic Neuroma and with Peripheral Vestibular Lesion by Human Posture Dynamics
- 1994
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Ingår i: Acta Oto-Laryngologica. - : Informa UK Limited. - 1651-2251 .- 0001-6489. ; 114:3, s. 479-483
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Tidskriftsartikel (refereegranskat)abstract
- A group of normal subjects (n = 17) was compared with two groups of patients, either with vestibular neuritis (n = 18) or with acoustic neuroma (n = 35) by means of posturography when stance was perturbed with vibrators attached to the calf muscles. Dynamic control of human posture was quantified by means of system identification, and the characteristic parameters of swiftness, stiffness, and damping of a transfer function from vibration to force platform response were used for further comparison. Fisher linear discriminant analysis was used to distinguish sets of parameters characteristic of each disease. Hence it was possible to distinguish the vestibular neuritis group both from the normal group (p < 0.01), and from the patients with acoustic neuroma (p < 0.001). The normal group was characterized by a different postural performance, with higher swiftness and stability parameters (stiffness, damping) than those of the patient groups. These findings indicate that there are differences in the dynamic control of posture between the two patient categories, which may require the development of differentiated rehabilitation programs.
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