| 1. |
- Akimov, D., et al.
(författare)
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Physics with WASA and PROMICE
- 1994
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Ingår i: Physics with GeV-particle beams, Juelich 1994. ; , s. 519-530
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Konferensbidrag (refereegranskat)
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| 2. |
- Calén, H., et al.
(författare)
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Deuteron breakup by 1.15 GeV protons and excitation of the Δ isobar
- 1993
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Ingår i: Physics Letters B. - : Elsevier. - 0370-2693 .- 1873-2445. ; 303:1-2, s. 10-15
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Tidskriftsartikel (refereegranskat)abstract
- The pd→npp reaction has been measured using circulating 1.15 GeV protons, a gas jet target and scintillation detectors selecting scattered protons with energy above 30 MeV. The distribution of the two-proton invariant mass has a maximum at 2.13 ± 0.01 GeV/c2 and a width of 130 ± 10 MeV/c2 (FWHM). We see no evidence of narrow (Γ < 30 MeV/c2) peaks in the phase-space region selected. The mass of X, excited in the pN→nX elementary process, is 40 MeV/c2 smaller than the mass of a real Δ isobar.
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| 3. |
- Calén, H, et al.
(författare)
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Deuteron breakup in the Δ-isobar region
- 1993
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Ingår i: Physica Scripta. - : Institute of Physics (IOP). - 0031-8949 .- 1402-4896. ; 48:1, s. 86-91
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Tidskriftsartikel (refereegranskat)abstract
- The pd → npp charge exchange reaction has been studied at 1.15 GeV incident proton energy in a selected kinematical region. The distributions of two-proton invariant mass Mpp, of angle of a single proton and the differential cross section dσ/dt of the (p, n) reaction are presented and compared with model predictions corrected for the acceptance of the experimental apparatus. We see no evidence of narrow (Γ < 30 MeV/c2) peaks in the Mpp interval 2.1-2.3 GeV/c2. The production of an intermediate Δ isobar in the primary (p, n) charge exchange and its subsequent absorption in a final state ΔN → pp process together with the background from quasi-free charge exchange scattering reasonably reproduces the general trends of the data.
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| 4. |
- Westergren-Thorsson, G, et al.
(författare)
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L-iduronate-rich glycosaminoglycans inhibit growth of normal fibroblasts independently of serum or added growth factors
- 1993
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Ingår i: Experimental Cell Research. - : Elsevier BV. - 0014-4827. ; 206:1, s. 9-93
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Tidskriftsartikel (refereegranskat)abstract
- The effects of various glycosaminoglycans (GAGs) on the growth rate of normal fibroblasts and a fibrosarcoma cell line (HT 1080) were examined. Cells were grown in 96-well microplates in the absence or presence of serum mitogens, epidermal (EGF), platelet-derived (PDGF), acidic fibroblast (aFGF), or basic fibroblast growth factor (bFGF). Cell number was measured by using crystal violet to stain cell nuclei (Westergren-Thorsson, G., Onnervik, P.-O., Fransson, L.-A., and Malmström, A. J. Cell. Phys. 147, 523-530, 1991) and also by using a Coulter counter. In the presence of serum mitogens, L-iduronate (IdoA)-rich GAGs, such as dermatan sulfate, heparin, and highly sulfated heparan sulfate, inhibited proliferation of normal cells (25-35%), whereas HT 1080 cells were unaffected or slightly stimulated. Ham's F-12 supplemented with insulin and transferrin but without growth factors was able to support growth of both cell types. Under these conditions, the IdoA-rich GAGs still suppressed growth of normal cells (40-55%), whereas HT 1080 cells again responded poorly. When growth factors were added proliferation of normal fibroblasts was further stimulated, EGF being the most effective. In the presence of either EGF, PDGF, or bFGF, IdoA-rich GAGs had a sustained inhibitory effect on normal fibroblasts (30-50% at concentrations at or above 10 micrograms/ml). However, in the presence of aFGF, both IdoA-rich and IdoA-poor heparan sulfates enhanced growth (nearly twofold after prolonged exposure) suggesting a stabilization of this growth factor. In general, IdoA-rich GAGs appear to inhibit proliferation of normal cells irrespective of the type of growth factor used. Therefore, GAGs are likely to act directly on cell-derived regulatory components, either before or after internalization. As fibrosarcoma cells were much less sensitive to growth inhibition, they may contain altered receptors for GAGs.
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| 5. |
- Carlstedt, I, et al.
(författare)
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Characterization of two different glycosylated domains from the insoluble mucin complex of rat small intestine.
- 1993
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Ingår i: The Journal of biological chemistry. - 0021-9258. ; 268:25, s. 18771-81
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Tidskriftsartikel (refereegranskat)abstract
- The highly glycosylated domains of rat small intestinal mucins were isolated after reduction and trypsin digestion and separated into two populations (A and B) by gel chromatography. The molecular mass values were 650 and 335 kDa, respectively, and the relative yields suggest that the two glycopeptides occur in equimolar proportions. Electron microscopy revealed linear structures with weight average lengths of 230 nm (A) and 110 nm (B) corresponding to a mass/unit length of about 3 kDa/nm. The protein cores (17-19%) contain large amounts of threonine (over 40%), serine (17-24%), and proline (18-19%). Carbohydrate and sulfate account for approximately 80 and 0.5%, respectively, and gas chromatography-mass spectrometry showed that the patterns of neutral and sialic acid-containing glycans are very similar in the two glycopeptides. Both contain a significant amount (7-10 mol %) of single GalNAc residues, the average oligosaccharide is about 4 sugar residues long, and the largest species observed are heptasaccharides. The major neutral and sialic acid-containing oligosaccharides are Fuc1-2Gal1-3GalNAcol and GlcNAc1-6(NeuGc2-Gal1-3)GalNAcol, respectively. Sialic acid is present as both N-acetyl- and N-glycoloyl-neuraminic acid. Repeated extractions of the tissue with guanidinium chloride left approximately 80% of the mucus glycoproteins as an insoluble glycoprotein complex whereas exposure to dithiothreitol or high speed homogenization accomplished complete solubilization. The "subunits" obtained after reduction with dithiothreitol are larger than glycopeptides A and B, and fragments corresponding in size to the latter are obtained after cleavage with trypsin. Most of the mucins from rat small intestine thus occurs as an insoluble glycoprotein complex composed of subunits joined with disulfide bonds. The subunits contain two highly glycosylated regions with different lengths substituted with very similar oligosaccharides.
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| 6. |
- Olsson, L E, et al.
(författare)
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MR imaging of absorbed dose distributions for radiotherapy using ferrous sulphate gels
- 1990
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Ingår i: Physics in Medicine and Biology. - : IOP Publishing. - 0031-9155 .- 1361-6560. ; 35:12, s. 31-1623
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Tidskriftsartikel (refereegranskat)abstract
- The measurement of absorbed dose distributions using dosemeter gel and magnetic resonance imaging (MRI) in a standard geometry has been investigated. Absorbed depth-dose curves and profiles measured with this new technique show good agreement with corresponding measurements using diodes. This was proven in a 60Co beam as well as an electron beam. The dosemeter gel is made of agarose and ferrous sulphate solution. The dose response is linear (r = 0.9996) in the investigated dose interval, 0-40 Gy. The sensitivity is a factor of about six higher compared to ordinary ferrous sulphate solution, known as 'Fricke'. This is a true 3D dose measurement technique which will have a number of applications in radiation therapy, since it is possible to mould the gel to arbitrary geometries, mix different radiation qualities and integrate the absorbed dose from different kinds of fields.
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| 7. |
- Westergren-Thorsson, G, et al.
(författare)
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Proliferation of cultured fibroblasts is inhibited by L-iduronate-containing glycosaminoglycans
- 1991
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Ingår i: Journal of Cellular Physiology. - : Wiley. - 0021-9541. ; 147:3, s. 30-523
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Tidskriftsartikel (refereegranskat)abstract
- We have modified a method (Gilles et al: Anal. Biochem., 159:109-113, 1986) for measuring cell number, that is based on the binding of crystal violet to cell nuclei and used it to assay effects of various glycosaminoglycans on growth of human lung fibroblasts. The procedure was modified by growing cells in microcultures (96 well microplates) and by measuring the amount of adsorbed dye using a microplate photometer after solubilisation of the cells with detergent. There was a linear relationship between absorbance and cell number measured by a Coulter Counter. The method is rapid and sensitive and can be used for screening many samples as well as measuring growth rates at low initial cell densities. Even the low growth rates obtained in the absence of serum can be detected. Human lung fibroblasts were growth arrested by serum deprivation and then grown for periods of up to 4 days in the presence of serum and exogenously added glycosaminoglycans (range, 0.1-100 micrograms/ml). Hyaluronan, chondroitin sulfate, and dextran sulfate were without effects, whereas dermatan sulfate, certain heparan sulfates, and heparin suppressed growth (20%-50% inhibition). The antiproliferative activity of dermatan sulfate increased with increasing iduronate content. Certain heparan sulfates, with moderately high sulfate and L-iduronate contents were better inhibitors than heparin despite the fact that the latter glycan has even higher sulfate and L-iduronate contents. The antiproliferative effect of exogenous glycans appeared after a lag period of 3-4 days, suggesting that they interfered with factors produced by the cells. Furthermore, the degree of inhibition was greater when the initial cell density was low. Above a certain level of seeded cells (approx. 10,000 cells/well), there was no inhibition by any of the glycosaminoglycans. It is possible that exogenous glycans cannot overcome endogenous growth-promoting effects in densely seeded cultures.
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| 8. |
- Westergren-Thorsson, G, et al.
(författare)
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TGF-beta enhances the production of hyaluronan in human lung but not in skin fibroblasts
- 1990
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Ingår i: Experimental Cell Research. - : Elsevier BV. - 0014-4827. ; 186:1, s. 5-192
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Tidskriftsartikel (refereegranskat)abstract
- Transforming growth factor-beta (TGF-beta) enhances the production of extracellular matrix components, such as type I and type III collagen, fibronectin, proteoglycans, in various cell types. The effect on hyaluronan synthesis in relation to proteoglycan synthesis has not been investigated. Human lung or skin fibroblast cultures were treated with TGF-beta in serum-free medium for various periods of time. 35SO4 or [3H]glucosamine was then added to the cultures in the absence of TGF-beta for up to 48 h. Hyaluronan and proteoglycans were isolated by ion-exchange chromatography and quantitated. TGF-beta induced a three- to fourfold increase in hyaluronan production by lung cells but had no effect on skin fibroblasts. In contrast, proteoglycan synthesis was enhanced in both cell types, although skin fibroblasts responded at lower concentrations of TGF-beta. Increased accumulation of hyaluronan was noted only in the cell medium, whereas proteoglycan accumulation was observed both in the medium and in the cell layer. The ED50 for TGF-beta on hyaluronan accumulation in lung cells was the same as that for proteoglycan accumulation, i.e., 40 pM. In skin fibroblasts the ED50 was considerably lower (4 pM). The induction time needed to attain full effect of TGF-beta was 6 h for both hyaluronan and proteoglycan synthesis. These results indicate that TGF-beta has tissue-specific effects on matrix production which may be of importance for control of cell proliferation in various disease states.
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| 9. |
- Gustafsson, L., et al.
(författare)
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A data acquisition system for the wide angle shower apparatus (WASA)
- 1993
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Ingår i: 1993 IEEE Conference Record Nuclear Science Symposium and Medical Imaging Conference. - 0780314875 ; , s. 518-521
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Konferensbidrag (refereegranskat)abstract
- A new data acquisition system based on concepts such as data switches and multiple-processors is described. The main topic is how data coming from a multicrate front-end in CAMAC, VME and FASTBUS are transported over different links to a buffer-matrix data switch and further into a farm of microprocessors. Modularity, scalability and multilevel data monitoring are important parts of the design goals that are presented. The system is intended for use in an experiment searching for rare events where high interaction rates are necessary and where a fast and selective trigger is difficult to define. Other experimental constraints, the trigger logical structure and the performance of the data acquisition are also described.
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| 10. |
- Gustafsson, L., et al.
(författare)
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A data acquisition system for the wide angle shower apparatus (WASA)
- 1994
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Ingår i: IEEE Transactions on Nuclear Science. - : Institute of Electrical and Electronics Engineers (IEEE). - 0018-9499 .- 1558-1578. ; 41:4, s. 1155-1158
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Tidskriftsartikel (refereegranskat)abstract
- A new data acquisition system based on concepts such as data switches and multiple-processors is described. The main topic is how data coming from a multicrate front-end in CAMAC, VME and FASTBUS are transported over different links to a buffer-matrix data switch and further into a farm of microprocessors. Modularity, scalability and multilevel data monitoring are important parts of the design goals that are presented. The system is intended for use in an experiment searching for rare events where high interaction rates are necessary and where a fast and selective trigger is difficult to define. Other experimental constraints, the trigger logical structure and the performance of the data acquisition are also described.
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