| 1. |
- Hesse, C, et al.
(författare)
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Measurement of apolipoprotein E (apoE) in cerebrospinal fluid
- 2000
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Ingår i: Neurochemical Research. - 1573-6903. ; 25:4, s. 511-517
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Tidskriftsartikel (refereegranskat)abstract
- Apolipoprotein E (apoE) is a protein involved in transport of lipids and has been implicated to play an important role in regeneration after nerve injury. Determination of apoE in cerebrospinal fluid (CSF) thus have a potential interest when studying different forms of brain damage and as a marker of ongoing regenerative processes in the brain. However, previous studies on CSF-ApoE in Alzheimer's disease (AD) have given inconclusive results. Such inconsistent results might be related to confounding factors interfering with sample handling and/or analyses, which have not been fully elucidated. We therefore examined different potential confounding factors for analyses of apoE in CSF and also developed a new enzyme linked immunosorbent assay (ELISA). The hydrophobic character of ApoE resulted in adsorption to different types of test tubes commonly used for collection of CSF at lumbar puncture, resulting in falsely low levels. This makes CSF handling critical, especially if samples are taken in different types of tubes, or is transferred to new tubes. Taking this confounding factors in consideration and analysing patient and control CSF handled in the same way and using the new ELISA, we could confirm our previous finding of reduced levels of ApoE in AD, (3.4 +/- 1.3 mg/l) compared with controls (4.5 +/- 2.7 mg/l) (p = 0.045). Both in the AD and in the control group, higher levels of CSF-ApoE was found in individuals possessing the ApoE4 alleles. Our results support that CSF-ApoE is reduced in AD, and that handling of CSF is a critical factor, which may explain the discrepant results from previous studies. Differences in the amount of patients and controls possessing the ApoE4 allele included might also increase the variance between different studies.
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| 2. |
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| 4. |
- Molander-Melin, M, et al.
(författare)
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Lipid analyses of human brain rafts
- 2003
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Ingår i: JOURNAL OF NEUROCHEMISTRY. - : Wiley. - 0022-3042 .- 1471-4159. ; 85, s. 34-34
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 5. |
- Nygren, C, et al.
(författare)
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Patients with Primary brain tumours have elevated serum titres of antibodies to the myelin glycolipid sulphatide
- 2001
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Ingår i: European neurology. - : S. Karger AG. - 0014-3022 .- 1421-9913. ; 45:1, s. 38-42
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Tidskriftsartikel (refereegranskat)abstract
- Primary and metastatic brain tumours may result in an altered exposure of normal cellular components to the immune system inducing an immune response measurable in autoantibodies. One potential immunogenic molecule is sulphatide, the major acidic glycolipid in myelin. Thirty-eight sera from 31 patients with primary and metastatic brain tumours have, therefore, been analyzed for the presence of antisulphatide antibodies by an ELISA performed on thin layer chromatography plates. Twenty-eight of the thirty-eight sera (74%) showed a positive antibody titre to sulphatide. The antibody titres were significantly higher (p < 0.01) in sera from patients with primary brain tumours than in sera from those with metastases. The study lends support to the possibility that antisulphatide antibodies could contribute to tissue damage and this might facilitate the invasive growth in primary brain tumours by demyelination. However, the pathogenic significance of these autoantibodies remains to be further elucidated.
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