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- Özgür, Erdogan, et al.
(författare)
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Porous functional nanofilms for designing bioinspired sensor surfaces
- 2015
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Ingår i: Proceeding of Advanced Materials World Congress. - Linköping, Sweden : VBRI Press. - 9789188252005
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Bio-mimicking of recognition features typical of biological molecules such as aminoacids, peptides, nucleic acid etc. is leading to the design and development of novel functional (artificial) materials for chemical/biochemical sensing applications. The insertion of biological molecules or their active sites into the backbone of synthetic polymers is one of the possible ways to achieve this. Herein, we synthesised a polymerisable derivative of an amino acid (L-histidine) through a set of reactions with 1-(1Hbenzo[d][1,2,3]triazol-1-yl)-2-methylprop-2-en-1-one (MA-Bt), since amino acid residues are the origin for the functional properties and highly selective substrate-binding ability of many extended biological structures. We obtained a functional monomer methacryloylamidohistidine (MAH), which was polymerised with 2-hydroxyethyl methacrylate in presence of polyvinyl alcohol (PVA) on a gold surface. The aim of using PVA was to obtain highly porous polymeric structure. For control purpose, polymers without MAH and PVA were also synthesised. The morphology of the polymeric film on gold surface was characterized with scanning electron microscopy (SEM) and atomic force microscopy (AFM). The obtained polymer showed significant affinity for Cu2+. The electrochemical behaviour of the polymeric films was systematically investigated with differential pulse voltammetry (DPV). The presence of pores was shown to significantly improve the recognition performance of the film.
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| 2. |
- Ahmad, T, et al.
(författare)
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Diabetic osteopathy and the IGF system in the Goto-Kakizaki rat
- 2008
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Ingår i: Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society. - : Elsevier BV. - 1096-6374. ; 18:5, s. 404-411
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Tidskriftsartikel (refereegranskat)
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| 3. |
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| 4. |
- Ahmad, Tashfeen, et al.
(författare)
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Peripheral quantitative computed tomography for the detection of diabetic osteopathy: a study in the Goto-Kakizaki rat.
- 2003
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Ingår i: Investigative radiology. - 0020-9996. ; 38:3, s. 171-6
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Tidskriftsartikel (refereegranskat)abstract
- RATIONALE AND OBJECTIVE: To assess the utility of dual energy x-ray absorptiometry (DEXA) and peripheral quantitative computed tomography (pQCT) in detecting trabecular and cortical bone changes in diabetes as a model of osteopenia. MATERIALS AND METHODS: The tibia from 10 type-2 diabetic Goto-Kakizaki (GK) rats and 10 control Wistar rats were analyzed by DEXA, pQCT, and ash weight determination. RESULTS: DEXA of GK rats showed a significant reduction in mineral content (32%) and density (24%) of the metaphysis, but not of the diaphysis. PQCT disclosed that the reduction of density predominantly pertained to the trabecular bone (reduced by 62%). Periosteal and endosteal circumferences of the diaphyses were increased and cortical thickness was unchanged leading to increased moment of inertia. CONCLUSIONS: This study suggests that in osteopathic conditions, cortical and trabecular bone should be separately examined within specific subregions to obtain relevant information. Loss of metaphyseal trabecular bone seems to be a predominant feature in diabetic rats. Moreover, there is increased moment of inertia in the diaphysis implying increased strength. These diagnostic features of diabetic osteopathy can only be assessed by pQCT. It may prove that similar changes occur in human type-2 diabetes, which could explain the susceptibility to periarticular fracture and Charcot arthropathy.
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| 6. |
- Rawle, Robert J., et al.
(författare)
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Detecting and Controlling Dye Effects in Single-Virus Fusion Experiments
- 2019
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Ingår i: Biophysical Journal. - : CELL PRESS. - 0006-3495 .- 1542-0086. ; 117:3, s. 445-452
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Tidskriftsartikel (refereegranskat)abstract
- Fluorescent dye-dequenching assays provide a powerful and versatile means to monitor membrane fusion events. They have been used in bulk assays, for measuring single events in live cells, and for detailed analysis of fusion kinetics for liposomal, viral, and cellular fusion processes; however, the dyes used also have the potential to perturb membrane fusion. Here, using single-virus measurements of influenza membrane fusion, we show that fluorescent membrane probes can alter both the efficiency and the kinetics of lipid mixing in a dye- and illumination-dependent manner. R18, a dye that is commonly used to monitor lipid mixing between membranes, is particularly prone to these effects, whereas Texas Red is somewhat less sensitive. R18 further undergoes photoconjugation to viral proteins in an illumination-dependent manner that correlates with its inactivation of viral fusion. These results demonstrate how fluorescent probes can perturb measurements of biological activity and provide both data and a method for determining minimally perturbative measurement conditions.
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| 10. |
- Goodman, Anna, et al.
(författare)
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Associations between birth characteristics and eating disorders across the life course : findings from 2 million males and females born in Sweden, 1975–1998
- 2014
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Ingår i: American Journal of Epidemiology. - : Oxford University Press (OUP). - 0002-9262 .- 1476-6256. ; 179:7, s. 852-863
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Tidskriftsartikel (refereegranskat)abstract
- Birth characteristics predict a range of major physical and mental disorders, but findings regarding eating disorders are inconsistent and inconclusive. This total-population Swedish cohort study identified 2,015,862 individuals born in 1975–1998 and followed them for anorexia nervosa, bulimia nervosa, and eating disorder not otherwise specified until the end of 2010. We examined associations with multiple family and birth characteristics and conducted within-family analyses to test for maternal-level confounding. In total, 1,019 males and 15,395 females received an eating disorder diagnosis. Anorexia nervosa was independently predicted by multiple birth (adjusted hazard ratio = 1.33, 95% confidence interval: 1.15, 1.53) for twins or triplets vs. singletons) and lower gestational age (adjusted hazard ratio = 0.96, 95% confidence interval: 0.95, 0.98) per extra week of gestation, with a clear dose-response pattern. Within-family analyses provided no evidence of residual maternal-level confounding. Higher birth weight for gestational age showed a strong, positive dose-response association with bulimia nervosa (adjusted hazard ratio = 1.15, 95% confidence interval: 1.09, 1.22, per each standard-deviation increase), again with no evidence of residual maternal-level confounding. We conclude that some perinatal characteristics may play causal, disease-specific roles in the development of eating disorders, including via perinatal variation within the normal range. Further research into the underlying mechanisms is warranted. Finally, several large population-based studies of anorexia nervosa have been conducted in twins; it is possible that these studies considerably overestimate prevalence.
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