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Träfflista för sökning "WFRF:(Fuchs A.) srt2:(2000-2004)"

Sökning: WFRF:(Fuchs A.) > (2000-2004)

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1.
  • Enfors, Sven-Olof, et al. (författare)
  • Physiological responses to mixing in large scale bioreactors
  • 2001
  • Ingår i: Journal of Biotechnology. - 0168-1656 .- 1873-4863. ; 85:2, s. 175-185
  • Tidskriftsartikel (refereegranskat)abstract
    • Escherichia coli fed-batch cultivations at 22 m(3) scale were compared to corresponding laboratory scale processes and cultivations using a scale-down reactor furnished with a high-glucose concentration zone to mimic the conditions in a feed zone of the large bioreactor. Formate accumulated in the large reactor, indicating the existence of oxygen limitation zones. It is suggested that the reduced biomass yield at large scale partly is due to repeated production/reassimilation of acetate from overflow metabolism and mixed acid fermentation products due to local moving zones with oxygen limitation. The conditions that generated mixed-acid fermentation in the scale-down reactor also induced a number of stress responses, monitored by analysis of mRNA of selected stress induced genes. The stress responses were relaxed when the cells returned to the substrate limited and oxygen sufficient compartment of the reactor. Corresponding analysis in the large reactor showed that the concentration of mRNA of four stress induced genes was lowest at the sampling port most distant from the feed zone. It is assumed that repeated induction/relaxation of stress responses in a large bioreactor may contribute to altered physiological properties of the cells grown in large-scale bioreactor. Flow cytometric analysis revealed reduced damage with respect to cytoplasmic membrane potential and integrity in cells grown in the dynamic environments of the large scale reactor and the scale-down reactor.
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  • Borg, A., et al. (författare)
  • Simultaneous velocity and concentration measurements in the near field of a turbulent low-pressure jet by digital particle image velocimetry-planar laser-induced fluorescence
  • 2001
  • Ingår i: Experiments in Fluids. - : Springer Science and Business Media LLC. - 0723-4864 .- 1432-1114. ; 31:2, s. 140-152
  • Tidskriftsartikel (refereegranskat)abstract
    • The main purpose of this work is to develop a method for simultaneous measurement of velocity and passive scalar concentration by means of digital particle image velocimetry and planar laser-induced fluorescence. Details of the implementation of the method are given, and the technique is applied to measurements of concentration and velocity in the centre-plane of a liquid jet with a Reynolds number of 6,000. The measurements are compared with large eddy simulations. Mean velocities and concentrations, fluctuating velocities and concentrations, and correlation between fluctuating velocities and concentrations. are analysed for the first six diameters downstream of the jet exit. The general agreement between measured and simulated results was found to be good, in particular for mean quantities. Mean profiles are also found to be in good agreement with other experimental work on jets reported in the literature. The whole-plane measurement method was found to be very useful for detailed comparisons of turbulent statistics with simulated data. The inadequacy of models for turbulent mass transport based on the standard gradient diffusion concept is demonstrated through the experimental data.
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  • Resultat 1-10 av 27

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