| 1. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 2. |
- Fujii, J., et al.
(författare)
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Identifying the Electronic Character and Role of the Mn States in the Valence Band of (Ga,Mn)As
- 2013
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Ingår i: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 111:9, s. 097201-
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Tidskriftsartikel (refereegranskat)abstract
- We report high-resolution hard x-ray photoemission spectroscopy results on (Ga,Mn)As films as a function of Mn doping. Supported by theoretical calculations we identify, for both low (1%) and high (13%) Mn doping values, the electronic character of the states near the top of the valence band. Magnetization and temperature-dependent core-level photoemission spectra reveal how the delocalized character of the Mn states enables the bulk ferromagnetic properties of (Ga,Mn)As.
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| 3. |
- Fujita, S., et al.
(författare)
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Spatial and temporal variability of snow accumulation rate on the East Antarctic ice divide between Dome Fuji and EPICA DML
- 2011
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Ingår i: The Cryosphere. - : Copernicus GmbH. - 1994-0416 .- 1994-0424. ; 5:4, s. 1057-1081
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Tidskriftsartikel (refereegranskat)abstract
- To better understand the spatio-temporal variability of the glaciological environment in Dronning Maud Land (DML), East Antarctica, a 2800-km-long Japanese-Swedish traverse was carried out. The route includes ice divides between two ice-coring sites at Dome Fuji and EPICA DML. We determined the surface mass balance (SMB) averaged over various time scales in the late Holocene based on studies of snow pits and firn cores, in addition to radar data. We find that the large-scale distribution of the SMB depends on the surface elevation and continentality, and that the SMB differs between the windward and leeward sides of ice divides for strong-wind events. We suggest that the SMB is highly influenced by interactions between the large-scale surface topography of ice divides and the wind field of strong-wind events that are often associated with high-precipitation events. Local variations in the SMB are governed by the local surface topography, which is influenced by the bedrock topography. In the eastern part of DML, the accumulation rate in the second half of the 20th century is found to be higher by similar to 15% than averages over longer periods of 722 a or 7.9 ka before AD 2008. A similar increasing trend has been reported for many inland plateau sites in Antarctica with the exception of several sites on the leeward side of the ice divides.
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| 4. |
- Butler, R, et al.
(författare)
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Uric acid stones in the urinary bladder of aryl hydrocarbon receptor (AhR) knockout mice
- 2012
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 1091-6490. ; 109:4, s. 1122-1126
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Tidskriftsartikel (refereegranskat)abstract
- The aryl hydrocarbon receptor (AhR) knockout mice raised in the laboratory of Fujii-Kuriyama have been under investigation for several years because of the presence in their urinary bladder of large, yellowish stones. The stones are composed of uric acid and become apparent in the bladders as tiny stones when mice are 10 wk of age. By the time the mice are 6 mo of age, there are usually two or three stones with diameters of 3–4 mm. The urate concentration in the serum was normal but in the urine the concentration was 40–50 mg/dL, which is 10 times higher than that in the WT littermates. There were no apparent histological pathologies in the kidney or joints and the levels of enzymes involved in elimination of purines were normal. The source of the uric acid was therefore judged to be from degradation of nucleic acids due to a high turnover of cells in the bladder itself. The bladder was fibrotic and the luminal side of the bladder epithelium was filled with eosinophilic granules. There was loss of E-cadherin between some epithelial cells, with an enlarged submucosal area filled with immune cells and sometimes invading epithelial cells. We hypothesize that in the absence of AhR there is loss of detoxifying enzymes, which leads to accumulation of unconjugated cytotoxins and carcinogens in the bladder. The presence of bladder toxins may have led to the increased apoptosis and inflammation as well as invasion of epithelial cells in the bladders of older mice.
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| 5. |
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| 6. |
- Fujii, Hitoshi, et al.
(författare)
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Interstitial Donor Codoping Method in (Ga, Mn)As to Increase Solubility of Mn and Curie Temperature
- 2011
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Ingår i: APPL PHYS EXPRESS. - : The Japan Society of Applied Physics (JSAP). - 1882-0778. ; 4:4
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Tidskriftsartikel (refereegranskat)abstract
- Based on first principles calculations, we propose a solubility control method of magnetic impurities in dilute magnetic semiconductors (DMSs). The low solubility of Mn in (Ga, Mn)As is experimentally and theoretically known. We show that donor atoms, such as Li, introduced at the interstitial sites in GaAs enhance the solubility of Mn. As a result, Mn can be doped to more than 20% in GaAs in the thermal equilibrium condition. The same effect can be seen when we dope Mn in GaAs with other interstitial donors, such as H, Na, K, Be, Mg, Ca, Cu, and Ag.
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| 7. |
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| 8. |
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| 9. |
- Ogawa, Y., et al.
(författare)
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On the statistical relation between ion upflow and naturally enhanced ion-acoustic lines observed with the EISCAT Svalbard radar
- 2011
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Ingår i: Journal of Geophysical Research. - 0148-0227 .- 2156-2202. ; 116, s. A03313-
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Tidskriftsartikel (refereegranskat)abstract
- We have investigated characteristics of ion upflow and naturally enhanced ion-acoustic lines (NEIALs) based on the European Incoherent Scatter (EISCAT) Svalbard radar (ESR) data continuously obtained between March 2007 and February 2008. For the ion upflow study we have used approximately 78,000 field-aligned profiles obtained with the ESR. For the NEIAL study we have identified approximately 1500 NEIALs in the ESR data at altitudes between 100 and 500 km. The occurrence frequency of ion upflow shows two peaks, at about 0800 and 1300 magnetic local time (MLT), while only one strong peak is seen around 0900 MLT for NEIALs. The upward ion flux also has only one peak around 1100-1300 MLT. The occurrence frequency of ion upflow varies strongly over season. It is higher in winter than in summer, whereas NEIALs are more frequent in summer than in winter. NEIALs frequently occur under high geomagnetic activity and also high solar activity conditions. Approximately 10% of NEIALs in the F region ionosphere were accompanied by NEIALs in the E region (occurred at altitudes below 200 km). About half of the E region enhanced echoes did not have an F region counterpart. Upshifted NEIALs dominate in the E region whereas downshifted NEIALs are usually stronger above an altitude of 300 km. The high occurrence frequency of NEIALs in the prenoon region (0800-1000 MLT) might be associated with acceleration of thermal ions to suprathermal ones. At the same MLT and geomagnetic latitude suprathermal ions and broadband extremely low frequency (BBELF) wave activity have been observed, according to previous studies.
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| 10. |
- Proletov, Ian, et al.
(författare)
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Primary and secondary glomerulonephritides 1.
- 2014
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Ingår i: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association. - : Oxford University Press (OUP). - 1460-2385. ; 29 Suppl 3:May, s. 186-200
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Tidskriftsartikel (refereegranskat)
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