| 1. |
- Göransson, J., et al.
(författare)
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Performance of a System for Rapid Phenotypic Antimicrobial Susceptibility Testing of Gram-Negative Bacteria Directly from Positive Blood Culture Bottles
- 2023
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Ingår i: Journal of Clinical Microbiology. - : American Society for Microbiology. - 0095-1137 .- 1098-660X. ; 61:3
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Tidskriftsartikel (refereegranskat)abstract
- The rapid administration of optimal antimicrobial treatment is paramount for the treatment of bloodstream infections (BSIs), and rapid antimicrobial susceptibility testing (AST) results are essential. Q-linea has developed the ASTar system, a rapid phenotypic AST device. Here, we report the performance of the ASTar BC G- (Gram-negative) kit when assessed according to the ISO 20776-2:2007 standard for performance evaluation of in vitro diagnostic AST devices. The evaluated ASTar BC G- kit uses a broad panel of 23 antimicrobials for the treatment of BSIs caused by Gram-negative fastidious and nonfastidious bacteria across a range of 6 to 14 2-fold dilutions, including cefoxitin as a screening agent for AmpC-producing Enterobacterales. The ASTar system processes blood culture samples to generate data on MICs and susceptible, intermediate, or resistant (SIR) category. The automated protocol includes concentration determination and concentration adjustment to enable a controlled inoculum, followed by broth microdilution (BMD) and microscopy performed continuously to generate MIC values within approximately 6 h once the test is run on the ASTar system. The performance of the ASTar system was assessed against the ISO 20776-2:2007 standard BMD reference method. Testing was performed across three sites, with results from 412 contrived blood cultures and 74 fresh clinical blood cultures. The ASTar system was also tested for reproducibility, with triplicate testing of 11 strains. The accuracy study comprised 8,650 data points of bacterium-antimicrobial tests. The ASTar system demonstrated an overall essential agreement (EA) of 95.8% (8,283/8,650) and a categorical agreement (CA) of 97.6% (8,433/8,639) compared to the reference BMD method. The overall rate of major discrepancies (MDs) was 0.9% (62/6,845), and that of very major discrepancies (VMDs) was 2.4% (30/1,239). This study shows that the ASTar system delivers reproducible results with overall EA and CA of >95%.
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| 2. |
- Strandberg, G., et al.
(författare)
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Bespoke climate indicators for the Swedish energy sector − a stakeholder focused approach
- 2024
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Ingår i: Climate Services. - Göteborg : IVL Svenska Miljöinstitutet. - 2405-8807. ; 34, s. 100486-100486
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Tidskriftsartikel (refereegranskat)abstract
- Climate change concerns the energy sector to a high degree because the sector is sensitive both to changing conditions for power and heat production, and to changing demand for electricity, heating and cooling. In this study potential consequences of climate change on different parts of the Swedish energy sector were assessed in a series of workshops, where climate and energy scientists, energy systems experts and analysts met with representativesof the energy sector to assess the vulnerability of the sector and consider what climate indicators could be used to assess impacts of relevance. The impact of climate change depends on the energy type. Hydropower, for which production is naturally linked to weather and climate, is significantly impacted by climate change. For other forms of production, such as nuclear power, other factors such as e.g. policy and technology development are more important. The series of workshops held in this study, where different aspects of climate change and consequences were discussed, proved very successful and has increased our understanding of climate impacts on the energy system.
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