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- Rybka, J., et al.
(författare)
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The use of ScCO2 for the extraction of LPS from S. enterica subsp. PCM 2266
- 2009
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Ingår i: Current Research Topics In Applied Microbiology And Microbial Biotechnology. ; , s. 460-464
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Konferensbidrag (refereegranskat)abstract
- The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO, flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO, flow rate and temperature were kept high (10g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effects within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used, while in the classical extraction method yield was less than 2%. All isolates were characterized by SDS-PAGE, by the spectra of the thiobarbituric acid reaction products and GLC-MS analysis.
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