1. |
|
|
2. |
- Barral, Anna-Maria, et al.
(författare)
-
Cell–cell adherence as a selection method for the generation of anti-melanoma monoclonal antibodies
- 1997
-
Ingår i: Journal of Immunological Methods. - 0022-1759. ; 203:1, s. 103-109
-
Tidskriftsartikel (refereegranskat)abstract
- The aim of the present study was to obtain monoclonal antibodies (mAbs) recognising human melanoma-associated antigens after immunisation of BALB/c mice with a 70–150 kDa membrane fraction from melanoma tumour tissues. Screening of specific antibody- producing hybridomas was performed using a novel cell–cell adherence method with the melanoma cell line M-14. Three mAbs of IgG1 isotype were selected: Mel-1, Mel-2 and Mel-3 which recognised the immunogen by ELISA and stained several melanoma cell lines positive in immunofluorescence. The molecular weight of the antigen was studied by different methods; a 170-kDa band was identified following immunoblotting of tumour lysate and a 72-kDa band was observed following immunoaffinity purification. Cell–cell adherence appears to be a reliable procedure for the generation of mAbs against native cellular antigens.
|
|
3. |
|
|