| 1. |
- Lovegrove, Jordan T., et al.
(författare)
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Assembly of Multicompartment Glycopolymer Worms in Aqueous Solution
- 2023
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Ingår i: Macromolecules. - 0024-9297. ; 56:8, s. 3195-3203
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Tidskriftsartikel (refereegranskat)abstract
- Hierarchical self-assembly is a versatile technique that allows the formation of many complex architectures on the nano- and microscale. However, many of these structures are formed in harsh organic solvents with nonbiocompatible polymers. Here, we investigate in more detail a synthetic path to produce biologically compatible, crosslinked, multicompartment worms (MCWs) in aqueous solution by incorporating thermoresponsive poly-N-isopropylacrylamide (PNIPAM) to direct assembly above the lower critical solution temperature (LCST). As the self-assembly into MCWs can be controlled externally by adjusting the temperature, we can provide new insight into the mechanism of hierarchical self-assembly. The growth of MCWs starting from primary micelles was observed above the LCST using in situ small-angle neutron scattering, dynamic light scattering, atomic force microscopy, and phase contrast microscopy.
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| 2. |
- Paracini, Nicolò, et al.
(författare)
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Structural Characterization of Nanoparticle-Supported Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron Scattering
- 2023
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Ingår i: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 15:3, s. 3772-3780
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Tidskriftsartikel (refereegranskat)abstract
- Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We combined scattering techniques with direct imaging, to gain access to sub-nanometer scale structural information on stable nanoparticle monolayers assembled on silicon crystals in a noncovalent manner using a Langmuir-Schaefer deposition. The structure of supported lipid bilayers formed on the nanoparticle arrays via vesicle fusion was investigated using a combination of grazing incidence X-ray and neutron scattering techniques complemented by fluorescence microscopy imaging. Ordered nanoparticle assemblies were shown to be suitable and stable substrates for the formation of curved and fluid lipid bilayers that retained lateral mobility, as shown by fluorescence recovery after photobleaching and quartz crystal microbalance measurements. Neutron reflectometry revealed the formation of high-coverage lipid bilayers around the spherical particles together with a flat lipid bilayer on the substrate below the nanoparticles. The presence of coexisting flat and curved supported lipid bilayers on the same substrate, combined with the sub-nanometer accuracy and isotopic sensitivity of grazing incidence neutron scattering, provides a promising novel approach to investigate curvature-dependent membrane phenomena on supported lipid bilayers.
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