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Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

Lomb, Lukas (author)
Barends, Thomas R. M. (author)
Kassemeyer, Stephan (author)
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Aquila, Andrew (author)
Epp, Sascha W. (author)
Erk, Benjamin (author)
Foucar, Lutz (author)
Hartmann, Robert (author)
Rudek, Benedikt (author)
Rolles, Daniel (author)
Rudenko, Artem (author)
Shoeman, Robert L. (author)
Andreasson, Jakob (author)
Uppsala universitet,Molekylär biofysik
Bajt, Sasa (author)
Barthelmess, Miriam (author)
Barty, Anton (author)
Bogan, Michael J. (author)
Bostedt, Christoph (author)
Bozek, John D. (author)
Caleman, Carl (author)
Coffee, Ryan (author)
Coppola, Nicola (author)
DePonte, Daniel P. (author)
Doak, R. Bruce (author)
Ekeberg, Tomas (author)
Uppsala universitet,Molekylär biofysik
Fleckenstein, Holger (author)
Fromme, Petra (author)
Gebhardt, Maike (author)
Graafsma, Heinz (author)
Gumprecht, Lars (author)
Hampton, Christina Y. (author)
Hartmann, Andreas (author)
Hauser, Guenter (author)
Hirsemann, Helmut (author)
Holl, Peter (author)
Holton, James M. (author)
Hunter, Mark S. (author)
Kabsch, Wolfgang (author)
Kimmel, Nils (author)
Kirian, Richard A. (author)
Liang, Mengning (author)
Maia, Filipe R. N. C. (author)
Uppsala universitet,Molekylär biofysik
Meinhart, Anton (author)
Marchesini, Stefano (author)
Martin, Andrew V. (author)
Nass, Karol (author)
Reich, Christian (author)
Schulz, Joachim (author)
Seibert, M. Marvin (author)
Uppsala universitet,Molekylär biofysik
Sierra, Raymond (author)
Soltau, Heike (author)
Spence, John C. H. (author)
Steinbrener, Jan (author)
Stellato, Francesco (author)
Stern, Stephan (author)
Timneanu, Nicusor (author)
Uppsala universitet,Molekylär biofysik
Wang, Xiaoyu (author)
Weidenspointner, Georg (author)
Weierstall, Uwe (author)
White, Thomas A. (author)
Wunderer, Cornelia (author)
Chapman, Henry N. (author)
Ullrich, Joachim (author)
Strüder, Lothar (author)
Schlichting, Ilme (author)
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 (creator_code:org_t)
2011
2011
English.
In: Physical Review B. Condensed Matter and Materials Physics. - 1098-0121 .- 1550-235X. ; 84:21, s. 214111-1-214111-6
Related links:
https://urn.kb.se/re...
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https://doi.org/10.1...
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  • Journal article (peer-reviewed)
Abstract Subject headings
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  • X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.

Subject headings

NATURVETENSKAP  -- Fysik (hsv//swe)
NATURAL SCIENCES  -- Physical Sciences (hsv//eng)

Publication and Content Type

ref (subject category)
art (subject category)

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