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Hepatitis B and C v...
Hepatitis B and C virus-induced hepatitis: apoptosis, autophagy and unfolded protein response.
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- Yeganeh, Behzad (author)
- University of Toronto, Toronto, Canada
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- Moghadam, Adel R (author)
- Islamic Azad University, Ardabil, Iran
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- Alizadeh, Javad (author)
- University of Manitoba, Winnipeg Canada
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- Wiechec, Emilia (author)
- Linköpings universitet,Avdelningen för neuro- och inflammationsvetenskap,Medicinska fakulteten
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- Alavian, Seyed M (author)
- Baqiyatallah Research Center for Gastroenterology and Liver Disease, Tehran, Iran
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- Hashemi, Mohammad (author)
- Zahedan University of Medical Sciences, Zahedan, Iran
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- Geramizadeh, Bita (author)
- Shiraz University of Medical Sciences, Shiraz, Iran
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- Samali, Afshin (author)
- National University of Ireland Galway, Galway, Ireland
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- Lankarani, Kamran B (author)
- Shiraz University of Medical Sciences, Shiraz, Iran
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- Post, Martin (author)
- University of Toronto, Toronto, Canada
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- Peymani, Payam (author)
- Shiraz University of Medical Sciences, Shiraz, Iran
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- Coombs, Kevin M (author)
- University of Manitoba, Winnipeg, Canada
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- Ghavami, Saeid (author)
- University of Manitoba, Winnipeg, Canada
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(creator_code:org_t)
- Pleasanton, CA, United States : Baishideng Publishing Group, 2015
- 2015
- English.
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In: World Journal of Gastroenterology. - Pleasanton, CA, United States : Baishideng Publishing Group. - 1007-9327 .- 2219-2840. ; 21:47, s. 13225-13239
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https://liu.diva-por... (primary) (Raw object)
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Abstract
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- AIM: To investigate the co-incidence of apoptosis, autophagy, and unfolded protein response (UPR) in hepatitis B (HBV) and C (HCV) infected hepatocytes.METHODS: We performed immunofluorescence confocal microscopy on 10 liver biopsies from HBV and HCV patients and tissue microarrays of HBV positive liver samples. We used specific antibodies for LC3β, cleaved caspase-3, BIP (GRP78), and XBP1 to detect autophagy, apoptosis and UPR, respectively. Anti-HCV NS3 and anti-HBs antibodies were also used to confirm infection. We performed triple blind counting of events to determine the co-incidence of autophagy (LC3β punctuate), apoptosis (cleaved caspase-3), and unfolded protein response (GRP78) with HBV and HCV infection in hepatocytes. All statistical analyses were performed using SPSS software for Windows (Version 16 SPSS Inc, Chicago, IL, United States). P-values < 0.05 were considered statistically significant. Statistical analyses were performed with Mann-Whitney test to compare incidence rates for autophagy, apoptosis, and UPR in HBV- and HCV-infected cells and adjacent non-infected cells.RESULTS: Our results showed that infection of hepatocytes with either HBV and HCV induces significant increase (P < 0.001) in apoptosis (cleavage of caspase-3), autophagy (LC3β punctate), and UPR (increase in GRP78 expression) in the HCV- and HBV-infected cells, as compared to non-infected cells of the same biopsy sections. Our tissue microarray immunohistochemical expression analysis of LC3β in HBVNeg and HBVPos revealed that majority of HBV-infected hepatocytes display strong positive staining for LC3β. Interestingly, although XBP splicing in HBV-infected cells was significantly higher (P < 0.05), our analyses show a slight increase of XBP splicing was in HCV-infected cells (P > 0.05). Furthermore, our evaluation of patients with HBV and HCV infection based on stage and grade of the liver diseases revealed no correlation between these pathological findings and induction of apoptosis, autophagy, and UPR.CONCLUSION: The results of this study indicate that HCV and HBV infection activates apoptosis, autophagy and UPR, but slightly differently by each virus. Further studies are warranted to elucidate the interconnections between these pathways in relation to pathology of HCV and HBV in the liver tissue.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
Keyword
- cell fate; cell death; hepatocyte; viral infection
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Yeganeh, Behzad
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Moghadam, Adel R
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Alizadeh, Javad
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Wiechec, Emilia
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Alavian, Seyed M
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Hashemi, Mohamma ...
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Geramizadeh, Bit ...
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Samali, Afshin
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Lankarani, Kamra ...
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Post, Martin
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Peymani, Payam
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Coombs, Kevin M
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Ghavami, Saeid
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Gastroenterology ...
- Articles in the publication
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World Journal of ...
- By the university
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Linköping University