SwePub
Sök i LIBRIS databas

  Utökad sökning

WFRF:(Go Michael R.)
 

Sökning: WFRF:(Go Michael R.) > (2006-2009) > Mass spectrometry r...

Mass spectrometry reveals specific and global molecular transformations during viral infection.

Go, Eden P (författare)
Wikoff, William R (författare)
Shen, Zhouxin (författare)
visa fler...
O'Maille, Grace (författare)
Morita, Hirotoshi (författare)
Conrads, Thomas P (författare)
Nordstrom, Anders (författare)
Department of Molecular Biology and The Center for Mass Spectrometry, The Scripps Research Institute
Trauger, Sunia A (författare)
Uritboonthai, Wilasinee (författare)
Lucas, David A (författare)
Chan, King C (författare)
Veenstra, Timothy D (författare)
Lewicki, Hanna (författare)
Oldstone, Michael B (författare)
Schneemann, Anette (författare)
Siuzdak, Gary (författare)
visa färre...
 (creator_code:org_t)
2006-08-17
2006
Engelska.
Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 5:9, s. 2405-16
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Mass spectrometry analysis was used to target three different aspects of the viral infection process: the expression kinetics of viral proteins, changes in the expression levels of cellular proteins, and the changes in cellular metabolites in response to viral infection. The combination of these methods represents a new, more comprehensive approach to the study of viral infection revealing the complexity of these events within the infected cell. The proteins associated with measles virus (MV) infection of human HeLa cells were measured using a label-free approach. On the other hand, the regulation of cellular and Flock House Virus (FHV) proteins in response to FHV infection of Drosophila cells was monitored using stable isotope labeling. Three complementary techniques were used to monitor changes in viral protein expression in the cell and host protein expression. A total of 1500 host proteins was identified and quantified, of which over 200 proteins were either up- or down-regulated in response to viral infection, such as the up-regulation of the Drosophila apoptotic croquemort protein, and the down-regulation of proteins that inhibited cell death. These analyses also demonstrated the up-regulation of viral proteins functioning in replication, inhibition of RNA interference, viral assembly, and RNA encapsidation. Over 1000 unique metabolites were also observed with significant changes in over 30, such as the down-regulated cellular phospholipids possibly reflecting the initial events in cell death and viral release. Overall, the cellular transformation that occurs upon viral infection is a process involving hundreds of proteins and metabolites, many of which are structurally and functionally uncharacterized.

Publikations- och innehållstyp

ref (ämneskategori)
art (ämneskategori)

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy