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Sökning: WFRF:(Gokhale P)

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  • Adewumi, Oluseun, et al. (författare)
  • Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
  • 2007
  • Ingår i: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1087-0156 .- 1546-1696. ; 25:7, s. 803-816
  • Tidskriftsartikel (refereegranskat)abstract
    • The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue- nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.
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  • Vijh, G., et al. (författare)
  • Solid freeform fabrication of aluminum alloy components : Numerical simulations
  • 2009
  • Ingår i: Transactions of the Indian Institute of Metals. - : Springer Science and Business Media LLC. - 0019-493X .- 0972-2815 .- 0975-1645. ; 62:4-5, s. 291-294
  • Tidskriftsartikel (refereegranskat)abstract
    • A heat transfer model has been developed to simulate the evolution of temperature during semi-solid metal (SSM) solid freeform fabrication (SFF) process. The governing equations were solved numerically using finite difference method. Deposition of simple cubic components of A356 (Al-7%Si-0.45%Mg) aluminum alloy have been simulated. The effect of process parameters such as deposition pattern, deposition velocity, slurry cross-section and slurry temperature on the transient temperature distribution in these components have been studied. Out of the three deposition patterns studied, i.e. rotating, alternating and unidirectional, the alternating pattern enabled depositing larger interfacefree components. Increasing the slurry flow rate resulted in the deposition of larger interface-free components
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  • Resultat 1-7 av 7

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