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N-glycans of Human ...
N-glycans of Human Protein C Inhibitor : Tissue-Specific Expression and Function
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- Sun, Wei, 1981- (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Sophia Schedin Weiss' group
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- Grassi, Paola (author)
- Division of Molecular Biosciences, Imperial College London,Anne Dell's group
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- Engström, Åke (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Sooriyaarachchi, Sanjeewani (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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- Ubhayasekera, Wimal (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Hreinsson, Julius, 1963- (author)
- Uppsala universitet,Institutionen för kvinnors och barns hälsa,Klinisk och experimentell reproduktionsbiologi/Olovsson
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- Wånggren, Kjell, 1954- (author)
- Uppsala universitet,Institutionen för kvinnors och barns hälsa,Klinisk och experimentell reproduktionsbiologi/Olovsson
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- Clark, Gary F (author)
- Department of Obstetrics, Gynecology and Women's Health, University of Missouri, Columbia, United States of America
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- Dell, Anne (author)
- Division of Molecular Biosciences, Imperial College London
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- Schedin-Weiss, Sophia (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Sophia Schedin Weiss' group
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(creator_code:org_t)
- 2011-12-19
- 2011
- English.
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In: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 6:12, s. e29011-
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Protein C inhibitor (PCI) is a serpin type of serine protease inhibitor that is found in many tissues and fluids in human, including blood plasma, seminal plasma and urine. This inhibitor displays an unusually broad protease specificity compared with other serpins. Previous studies have shown that the N-glycan(s) and the NH(2)-terminus affect some blood-related functions of PCI. In this study, we have for the first time determined the N-glycan profile of seminal plasma PCI, by mass spectrometry. The N-glycan structures differed markedly compared with those of both blood-derived and urinary PCI, providing evidence that the N-glycans of PCI are expressed in a tissue-specific manner. The most abundant structure (m/z 2592.9) had a composition of Fuc(3)Hex(5)HexNAc(4), consistent with a core fucosylated bi-antennary glycan with terminal Lewis x. A major serine protease in semen, prostate specific antigen (PSA), was used to evaluate the effects of N-glycans and the NH(2)-terminus on a PCI function related to the reproductive tract. Second-order rate constants for PSA inhibition by PCI were 4.3 +/- 0.2 and 4.1 +/- 0.5 M(-1) s(-1) for the natural full-length PCI and a form lacking six amino acids at the NH(2)-terminus, respectively, whereas these constants were 4.8 +/- 0.1 and 29 +/- 7 M(-1) s(-1) for the corresponding PNGase F-treated forms. The 7-8-fold higher rate constants obtained when both the N-glycans and the NH(2-)terminus had been removed suggest that these structures jointly affect the rate of PSA inhibition, presumably by together hindering conformational changes of PCI required to bind to the catalytic pocket of PSA.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- N-glycosylation
- Prostate specific antigen
- Protein C inhibitor
- Seminal plasma
- Serpin
- Biochemistry
- Biokemi
- Biokemi
- Biochemistry
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Sun, Wei, 1981-
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Grassi, Paola
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Engström, Åke
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Sooriyaarachchi, ...
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Ubhayasekera, Wi ...
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Hreinsson, Juliu ...
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Wånggren, Kjell, ...
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Clark, Gary F
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Dell, Anne
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Schedin-Weiss, S ...
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PLOS ONE
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Uppsala University