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Affinity Capillary ...
Affinity Capillary Electrophoresis as a Tool To Characterize Molecularly Imprinted Nanogels in Solution
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- Contardi, Cecilia (författare)
- Department of Drug Sciences, University of Pavia, 27100 Pavia, PV, Italy
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- Rubes, Davide (författare)
- Department of Drug Sciences, University of Pavia, 27100 Pavia, PV, Italy
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- Serra, Massimo (författare)
- Department of Drug Sciences, University of Pavia, 27100 Pavia, PV, Italy
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- Dorati, Rossella (författare)
- Department of Drug Sciences, University of Pavia, 27100 Pavia, PV, Italy
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- Dattilo, Marco (författare)
- Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Arcavacata di Rende, CS, Italy
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- Mavliutova, Liliia (författare)
- Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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- Patrini, Maddalena (författare)
- Department of Physics, University of Pavia, 27100 Pavia, PV, Italy
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- Guglielmann, Raffaella (författare)
- Department of Mathematics F. Casorati, University of Pavia, 27100 Pavia, PV, Italy
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- Sellergren, Börje (författare)
- Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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- De Lorenzi, Ersilia (författare)
- Department of Drug Sciences, University of Pavia, 27100 Pavia, PV, Italy
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(creator_code:org_t)
- American Chemical Society (ACS), 2024
- 2024
- Engelska.
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 96:7, s. 3017-3024
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In this work, an innovative and accurate affinity capillary electrophoresis (ACE) method was set up to monitor the complexation of aqueous MIP nanogels (NGs) with model cancer-related antigens. Using α2,6′- and α2,3′-sialyllactose as oversimplified cancer biomarker-mimicking templates, NGs were synthesized and characterized in terms of size, polydispersity, and overall charge. A stability study was also carried out in order to select the best storage conditions and to ensure product quality. After optimization of capillary electrophoresis conditions, injection of MIP NGs resulted in a single, sharp, and efficient peak. The mobility shift approach was applied to quantitatively estimate binding affinity, in this case resulting in an association constant of K ≈ 106 M–1. The optimized polymers further displayed a pronounced discrimination between the two sialylated sugars. The newly developed ACE protocol has the potential to become a very effective method for nonconstrained affinity screening of NG in solution, especially during the NG development phase and/or for a final accurate quantitation of the observed binding.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- polymer nanoparticles
- sialic-acid
- expression
- beta(2)-microglobulin
- glycosylation
- adsorption
- isotherm
- gold
Publikations- och innehållstyp
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- art (ämneskategori)
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Contardi, Cecili ...
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Rubes, Davide
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Serra, Massimo
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Dorati, Rossella
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Dattilo, Marco
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Mavliutova, Lili ...
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Patrini, Maddale ...
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Guglielmann, Raf ...
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Sellergren, Börj ...
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De Lorenzi, Ersi ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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Analytical Chemi ...
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Malmö universitet