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- Höök, Fredrik, 1966, et al.
(författare)
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Supported lipid bilayers, tethered lipid vesicles, and vesicle fusion investigated using gravimetric, plasmonic, and microscopy techniques
- 2007
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Ingår i: Biointerphases. - : American Vacuum Society. - 1559-4106 .- 1934-8630. ; 3:2
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Tidskriftsartikel (refereegranskat)abstract
- This article summarizes our most recent contributions to the rapidly growing field of supported lipid assemblies with emphasis on current studies addressing both fundamental and applied aspects of supported lipid bilayer (SLB) and tethered lipid vesicles (TLVs) to be utilized in sensing applications. The new insights obtained from combining the quartz crystal microbalance with dissipation monitoring technique with surface plasmon resonance are described, and we also present recent studies in which nanoplasmonic sensing has been used in studies of SLBs and TLVs. To gain full control over the spatial arrangement of TLVs in both two and three dimensions, we have developed a method for site-selective and sequence-specific sorting of DNA-tagged vesicles to surfaces modified with complementary DNA. The combination of this method with nanoplasmonic sensing formats is covered as well as the possibility of using DNA-modified vesicles for the detection of unlabeled DNA targets on the single-molecule level. Finally, a new method for membrane fusion induced by hybridization of vesicle-anchored DNA is demonstrated, including new results on content mixing obtained with vesicle populations encapsulating short, complementary DNA strands.
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| 2. |
- Gunnarsson, Anders, 1981, et al.
(författare)
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Kinetic and thermodynamic characterization of single-mismatch discrimination using single-molecule imaging
- 2009
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Ingår i: Nucleic Acids Research. - : Oxford University Press (OUP). - 0305-1048 .- 1362-4962. ; 37:14, s. e99 (art no)-
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Tidskriftsartikel (refereegranskat)abstract
- A single-molecule detection setup based on total internal reflection fluorescence (TIRF) microscopy has been used to investigate association and dissociation kinetics of unlabeled 30mer DNA strands. Single-molecule sensitivity was accomplished by letting unlabeled DNA target strands mediate the binding of DNA-modified and fluorescently labeled liposomes to a DNA-modified surface. The liposomes, acting as signal enhancer elements, enabled the number of binding events as well as the residence time for high affinity binders (K-d
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| 4. |
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| 5. |
- Markström, Martin, 1976, et al.
(författare)
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Dynamic microcompartmentalization of giant unilamellar vesicles by sol gel transition and temperature induced shrinking/swelling of poly(N-isopropyl acrylamide)
- 2007
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Ingår i: Soft Matter. - : Royal Society of Chemistry (RSC). - 1744-6848 .- 1744-683X. ; 3:5, s. 587-595
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Tidskriftsartikel (refereegranskat)abstract
- Giant unilamellar vesicles (GUVs) were microinjected with aqueous solutions of poly(N-isopropyl acrylamide) (PNIPAAm). Temperature-dependent sol-gel phase transitions of the solutions, followed by shrinking and swelling of the resulting hydrogel, were studied in the presence of a variety of co-solutes within the GUV. Reversible formation of a dense, spherical hydrogel structure (compartment) was observed in all cases with defined shrinking/swelling behaviour at temperatures above the lower critical solution temperatures (LCSTs). Nanotube-mediated merging of two vesicles with thus formed compartments resulted in a single GUV with two internalized hydrogel structures. As an application example, we demonstrate how fluorescent nanoparticles can be immobilized in such gel structures.
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