| 1. |
- Guo, Di, et al.
(författare)
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Cholecystokinin-like peptide mediates satiety by inhibiting sugar attraction
- 2021
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Ingår i: PLOS Genetics. - : Public Library of Science (PLoS). - 1553-7390 .- 1553-7404. ; 17:8
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Tidskriftsartikel (refereegranskat)abstract
- Feeding is essential for animal survival and reproduction and is regulated by both internal states and external stimuli. However, little is known about how internal states influence the perception of external sensory cues that regulate feeding behavior. Here, we investigated the neuronal and molecular mechanisms behind nutritional state-mediated regulation of gustatory perception in control of feeding behavior in the brown planthopper and Drosophila. We found that feeding increases the expression of the cholecystokinin-like peptide, sulfakinin (SK), and the activity of a set of SK-expressing neurons. Starvation elevates the transcription of the sugar receptor Gr64f and SK negatively regulates the expression of Gr64f in both insects. Interestingly, we found that one of the two known SK receptors, CCKLR-17D3, is expressed by some of Gr64f-expressing neurons in the proboscis and proleg tarsi. Thus, we have identified SK as a neuropeptide signal in a neuronal circuitry that responds to food intake, and regulates feeding behavior by diminishing gustatory receptor gene expression and activity of sweet sensing GRNs. Our findings demonstrate one nutritional state-dependent pathway that modulates sweet perception and thereby feeding behavior, but our experiments cannot exclude further parallel pathways. Importantly, we show that the underlying mechanisms are conserved in the two distantly related insect species.
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| 2. |
- Qiu, X. T., et al.
(författare)
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Transcriptomic and proteomic profiling of the anterior cingulate cortex in neuropathic pain model rats
- 2023
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Ingår i: Frontiers in Molecular Neuroscience. - 1662-5099. ; 16
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Tidskriftsartikel (refereegranskat)abstract
- BackgroundNeuropathic pain (NP) takes a heavy toll on individual life quality, yet gaps in its molecular characterization persist and effective therapy is lacking. This study aimed to provide comprehensive knowledge by combining transcriptomic and proteomic data of molecular correlates of NP in the anterior cingulate cortex (ACC), a cortical hub responsible for affective pain processing. MethodsThe NP model was established by spared nerve injury (SNI) in Sprague-Dawley rats. RNA sequencing and proteomic data from the ACC tissue isolated from sham and SNI rats 2 weeks after surgery were integrated to compare their gene and protein expression profiles. Bioinformatic analyses were performed to figure out the functions and signaling pathways of the differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) enriched in. ResultsTranscriptomic analysis identified a total of 788 DEGs (with 49 genes upregulated) after SNI surgery, while proteomic analysis found 222 DEPs (with 89 proteins upregulated). While Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of the DEGs suggested that most of the altered genes were involved in synaptic transmission and plasticity, bioinformatics analysis of the DEPs revealed novel critical pathways associated with autophagy, mitophagy, and peroxisome. Notably, we noticed functionally important NP-related changes in the protein that occurred in the absence of corresponding changes at the level of transcription. Venn diagram analysis of the transcriptomic and proteomic data identified 10 overlapping targets, among which only three genes (XK-related protein 4, NIPA-like domain-containing 3, and homeodomain-interacting protein kinase 3) showed concordance in the directions of change and strong correlations between mRNA and protein levels. ConclusionThe present study identified novel pathways in the ACC in addition to confirming previously reported mechanisms for NP etiology, and provided novel mechanistic insights for future research on NP treatment. These findings also imply that mRNA profiling alone fails to provide a complete landscape of molecular pain in the ACC. Therefore, explorations of changes at the level of protein are necessary to understand NP processes that are not transcriptionally modulated.
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| 3. |
- Zhou, Feifei, et al.
(författare)
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ENSO weakens the co-variability between the spring persistent rains and Asian summer monsoon: Evidences from tree-ring data in southeastern China
- 2024
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Ingår i: Journal of Hydrology. - 0022-1694. ; 634
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Tidskriftsartikel (refereegranskat)abstract
- The Spring Persistent Rains (SPR) and the Asian Summer Monsoon (ASM) are the two dominant rainfall systems in East Asia, providing together a majority of annual rainfall in southeastern China (SEC). Since observational data in SEC were mostly unavailable until the 1950s, proxy records that are capable of capturing the SPR and ASM variations are required to examine the long-term co-variability patterns between them. Tree-ring earlywood and latewood δ18O records in SEC were found to respond to relative humidity (RH) during the SPR and ASM seasons, respectively, allowing us, for the first time, to reconstruct the RH changes of SPR and ASM back to 1801. The two reconstructions can explain 44.9 % and 42.3 % of the instrumental variance. We observed a long-lasting wet epoch in the 1920s–60s for both the SPR and ASM, caused by a peak in the land–ocean thermal contrast. The El Niño-Southern Oscillation (ENSO) and the Intertropical Convergence Zone (ITCZ) were found to be the two leading tropical systems that modulated the SPR and ASM co-variability. During a period with weakened ENSO variance, the RH of SPR and ASM showed in-phase changes driven by the ITCZ. However, when the ENSO variance became strengthened, the co-variability collapsed since the ENSO can offset the influence of the ITCZ via teleconnections.
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