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- Andersson, Anders, et al.
(författare)
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A transcriptional timetable of autumn senescence
- 2004
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Ingår i: Genome Biology. - : Springer Science and Business Media LLC. - 1465-6906 .- 1474-760X. ; 5:4, s. R24-
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Tidskriftsartikel (refereegranskat)abstract
- Background We have developed genomic tools to allow the genus Populus (aspens and cottonwoods) to be exploited as a full-featured model for investigating fundamental aspects of tree biology. We have undertaken large-scale expressed sequence tag (EST) sequencing programs and created Populus microarrays with significant gene coverage. One of the important aspects of plant biology that cannot be studied in annual plants is the gene activity involved in the induction of autumn leaf senescence. Results On the basis of 36,354 Populus ESTs, obtained from seven cDNA libraries, we have created a DNA microarray consisting of 13,490 clones, spotted in duplicate. Of these clones, 12,376 (92%) were confirmed by resequencing and all sequences were annotated and functionally classified. Here we have used the microarray to study transcript abundance in leaves of a free-growing aspen tree (Populus tremula) in northern Sweden during natural autumn senescence. Of the 13,490 spotted clones, 3,792 represented genes with significant expression in all leaf samples from the seven studied dates. Conclusions We observed a major shift in gene expression, coinciding with massive chlorophyll degradation, that reflected a shift from photosynthetic competence to energy generation by mitochondrial respiration, oxidation of fatty acids and nutrient mobilization. Autumn senescence had much in common with senescence in annual plants; for example many proteases were induced. We also found evidence for increased transcriptional activity before the appearance of visible signs of senescence, presumably preparing the leaf for degradation of its components.
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| 5. |
- Gustafsson Lundberg, Johanna
(författare)
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Kyrka och kön – om könskonstruktioner i Svenska kyrkan 1945-1985
- 2001
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The aim of this thesis is to analyse how sex and gender have been constructed within the context of the Swedish Lutheran Church 1954-1985. The studied texts mainly consist of Christian pastoral work primarily dealing with sexuality and marriage. But the material also includes investigations within this field published by the Swedish state (SOU), and newspaper articles where marriage and sexuality are discussed from or against a Christian point of view. The question about how sex and gender is constructed have been explored by using three different analytic themes influenced by gender theory: a/ sexuality and gender, b/ Christian views on marriage and married life and c/ the relation between tradition and modernity. These themes highlight in different ways aspects of the construction of sex and gender, e.g., the relation between the individual and social structures, questions concerning private and public spheres, and different views on the person within tradition and modernity. Another important aspect concerns power, namely a discursive interpretation of power and a substantial one. Both concepts illustrate the construction of sex and gender in important ways. During the first period, 1945-1959, there is an emphasis on difference in the construction of sex and gender. By some, man and woman are viewed as two incommensurable entities. During the 1960s an indivdualist perspective is offered. This attaches great significance to feelings and to the development and maturity of the individual and the inner self. Within this perspective the category of gender becomes subordinated. In the 1970s a new perspective on theology is formulated by feminist theologians. Gender serves in a feminist theological approach as a structural critique. Gender as a category is made into a total perspective through which a gender perspective is applied to different areas in society. The question about homosexuality challenges in 1985 a traditional dichotomic construction of sex and gender in which woman and man are constructed as two polarities who are attracted to each other. A queer theoretical perspective highlights how gender - by being constructed from a heterosexual point of view - also includes a strict heterosexual understanding of sexuality. In concluding the thesis I discuss how the Swedish Lutheran Church can be understood as a value tradition in which one finds many vying moral views on sex and gender.
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| 6. |
- Gustafsson Lundberg, Johanna
(författare)
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Kyrkan och sexualiteten
- 2004
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Ingår i: Svensk Kyrkotidning. - 0346-2153. ; 100:27-28, s. 373-377
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 8. |
- Gustafsson Lundberg, Johanna, et al.
(författare)
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Listiga kvinnor och driftsdrivna män
- 2002
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Ingår i: Genus, stigma och religion. - 9144022972 ; , s. 127-145
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Bokkapitel (refereegranskat)
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| 9. |
- Miranda-Vizuete, Antonio, et al.
(författare)
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Characterization of Sptrx, a novel member of the thioredoxin family specifically expressed in human spermatozoa
- 2001
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Ingår i: Journal of Biological Chemistry. - : American Society for Biochemistry and Molecular Biology. - 0021-9258 .- 1083-351X. ; 276:34, s. 31567-31574
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Tidskriftsartikel (refereegranskat)abstract
- Thioredoxins (Trx) are small ubiquitous proteins that participate in different cellular processes via redox-mediated reactions. We report here the identification and characterization of a novel member of the thioredoxin family in humans, named Sptrx (sperm-specific trx), the first with a tissue-specific distribution, located exclusively in spermatozoa. Sptrx open reading frame encodes for a protein of 486 amino acids composed of two clear domains: an N-terminal domain consisting of 23 highly conserved repetitions of a 15-residue motif and a C-terminal domain typical of thioredoxins. Northern analysis and in situ hybridization shows that Sptrx mRNA is only expressed in human testis, specifically in round and elongating spermatids. Immunostaining of human testis sections identified Sptrx protein in spermatids, while immunofluorescence and immunogold electron microscopy analysis demonstrated Sptrx localization in the cytoplasmic droplet of ejaculated sperm. Sptrx appears to have a multimeric structure in native conditions and is able to reduce insulin disulfide bonds in the presence of NADPH and thioredoxin reductase. During mammalian spermiogenesis in testis seminiferous tubules and later maturation in epididymis, extensive reorganization of disulfide bonds is required to stabilize cytoskeletal sperm structures. However, the molecular mechanisms that control these processes are not known. The identification of Sptrx with an expression pattern restricted to the postmeiotic phase of spermatogenesis, when the sperm tail is organized, suggests that Sptrx might be an important factor in regulating critical steps of human spermiogenesis.
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| 10. |
- Movérare, Sofia, et al.
(författare)
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Estren is a selective estrogen receptor modulator with transcriptional activity.
- 2003
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Ingår i: Molecular pharmacology. - : American Society for Pharmacology & Experimental Therapeutics (ASPET). - 0026-895X .- 1521-0111. ; 64:6, s. 1428-33
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Tidskriftsartikel (refereegranskat)abstract
- It was recently reported that the synthetic compound estren increases bone mass without affecting reproductive organs or classic transcription. The aim of the present study was to further characterize the in vivo and in vitro effects of estren. We demonstrate that estren is a selective estrogen receptor modulator (SERM) with a strong effect on thymus, a moderate effect on uterus and trabecular bone, but no major effect on fat or cortical bone in 11-month-old ovariectomized mice. The effect of estren on trabecular bone and uterus is mediated via estrogen receptors (ERs) because no effect is seen in ER double-inactivated mice. Furthermore, with the use of ERalpha- and ERbeta-expressing reporter cell lines, we demonstrate that estren displays an agonistic effect on transcriptional activity of an estrogen-responsive element-driven reporter gene with a degree of agonism similar to that of 17beta-estradiol for both ERalpha and ERbeta. Thus, estren has the capacity to exert genomic effects via both ERalpha and ERbeta. We conclude, in contrast to what was previously reported by others, that estren is a SERM with transcriptional activity.
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