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| 2. |
- Hill-Pearce, R. E., et al.
(författare)
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The effect of bilayer regions on the response of epitaxial graphene devices to environmental gating
- 2015
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Ingår i: Carbon. - : PERGAMON-ELSEVIER SCIENCE LTD. - 0008-6223 .- 1873-3891. ; 93, s. 896-902
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Tidskriftsartikel (refereegranskat)abstract
- The effect of a bilayer area on the electronic response to environmental gating of a monolayer graphene Hall bar device is investigated using room temperature magnetotransport and scanning Kelvin probe microscopy measurements in a controlled environment. The device is tuned through the charge neutrality point with n-p-n-junctions formed. Scanning Kelvin probe measurements show that the work function of the monolayer graphene decreases more than that of the bilayer area however magnetotransport measurements show a larger change in carrier concentration for bilayer graphene with environmental gating. Interface scattering at the boundary between the monolayer and bilayer regions also affects device response with field-dependent suppression of the conductivity observed near the charge neutrality point. Simultaneous electronic and environmental scanning Kelvin probe measurements are used to build nano-scale maps of the work function of the device surface revealing the areas of greatest work function change with environmental gating. Crown Copyright (C) 2015 Published by Elsevier Ltd. All rights reserved.
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| 3. |
- Hill, Sandra Malmgren, 1987, et al.
(författare)
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Asymmetric Inheritance of Aggregated Proteins and Age Reset in Yeast Are Regulated by Vac17-Dependent Vacuolar Functions
- 2016
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Ingår i: Cell Reports. - : Elsevier BV. - 2211-1247. ; 16:3, s. 826-838
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Tidskriftsartikel (refereegranskat)abstract
- Age can be reset during mitosis in both yeast and stem cells to generate a young daughter cell from an aged and deteriorated one. This phenomenon requires asymmetry-generating genes (AGGs) that govern the asymmetrical inheritance of aggregated proteins. Using a genome-wide imaging screen to identify AGGs in Saccharomyces cerevisiae, we discovered a previously unknown role for endocytosis, vacuole fusion, and the myosin-dependent adaptor protein Vac17 in asymmetrical inheritance of misfolded proteins. Overproduction of Vac17 increases deposition of aggregates into cytoprotective vacuole-associated sites, counteracts age-related breakdown of endocytosis and vacuole integrity, and extends replicative lifespan. The link between damage asymmetry and vesicle trafficking can be explained by a direct interaction between aggregates and vesicles. We also show that the protein disaggregase Hsp104 interacts physically with endocytic vesicle-associated proteins, such as the dynamin-like protein, Vps1, which was also shown to be required for Vac17-dependent sequestration of protein aggregates. These data demonstrate that two physiognomies of aging-reduced endocytosis and protein aggregation-are interconnected and regulated by Vac17.
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| 4. |
- Yang, Xinping, et al.
(författare)
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Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing
- 2016
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Ingår i: Cell. - : Elsevier BV. - 0092-8674 .- 1097-4172. ; 164:4, s. 805-817
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Tidskriftsartikel (refereegranskat)abstract
- While alternative splicing is known to diversify the functional characteristics of some genes, the extent to which protein isoforms globally contribute to functional complexity on a proteomic scale remains unknown. To address this systematically, we cloned full-length open reading frames of alternatively spliced transcripts for a large number of human genes and used protein-protein interaction profiling to functionally compare hundreds of protein isoform pairs. The majority of isoform pairs share less than 50% of their interactions. In the global context of interactome network maps, alternative isoforms tend to behave like distinct proteins rather than minor variants of each other. Interaction partners specific to alternative isoforms tend to be expressed in a highly tissue-specific manner and belong to distinct functional modules. Our strategy, applicable to other functional characteristics, reveals a widespread expansion of protein interaction capabilities through alternative splicing and suggests that many alternative "isoforms'' are functionally divergent (i.e., "functional alloforms'').
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